[18p-E302-1~11] 12.6 Nanobiotechnology

[18p-E302-5] Detection of CNT-specific peptide binding to carbon electrodes.

〇Ichiro Yamashita¹, Yuri Shimizu¹, Naofumi Okamoto², Huanwen Han¹, Tingche Chu¹, Masakazu Nakamura² (1.Osaka Univ., 2.NAIST)

Keywords:CNT-binding peptide, Aptamer, Electrochemical measurement

Using phage display, we have obtained 12 amino acid peptide aptamers that adsorb on CNTs. We also modify these peptides on the surface of cage-shaped proteins. We have previously reported that it is possible to measure their adsorption using Electrochemical Impedance Spectroscopy (EIS). Now, we tried to detect the adsorption of these peptides on a screen-printed carbon electrode using Cyclic Voltammogram (CV). It was demonstrated that adsorption could be quantitatively evaluated from the difference in redox peak potentials and the peak current values.