[XIV-29-04_06] 畜産物利用（XIV-午前）

[XIV29-05E] Microarray analysis of bovine mammary epithelial cells stimulated with mastitis pathogens

^○Takagi Michihiro^1,2, Komatsu Ryoya^1,2,3, Villena Julio⁴, Albarracin Leonardo⁴, Islam Md. Aminul^1,2, Suda Yoshihito⁵, Ohtsubo Wakako Ikeda^1,2,3, Nochi Tomonori^1,2,3, Aso Hisashi^1,2,3, Kitazawa Haruki^1,2,3 (1.Tohoku Univ. (Agri.), 2.CFAI, 3.JSPS Core-to-Core Program, 4.CERELA-CONICET, 5.Miyagi Univ.)

[Objective] Bovine mastitis is a one of most economically devastating diseases in dairy cattle, resulting from a complex host-pathogen interactions. This study was to identify the transcriptome alterations associated with innate immune responses to mastitis caused by E. coli and S. aureus infection.
[Methods] Bovine mammary epithelial cells were stimulated with either LPS or two strains of S. aureus for 12 h and the transcriptome profiles were generated by using the Bovine (V2) Gene Expression Microarray. Selected deregulated genes including toll-like receptors 1-9 (TLR 1-9) were quantified by RT-qPCR .
[Results] A total of 429, 520 and 465 genes were differentially expressed after LPS, S. aureus strains stimulation, respectively. Expression of TLR 1-9 were confirmed. LPS-induced up regulated genes are found to be related to immune responses. Identified genes could be useful for evaluation of immunobiotics for mastitis control.