[XIV-29-04_06] 畜産物利用（XIV-午前）

[XIV29-04E] Selection of wakame utilizing lactobacilli adhering to porcine intestinal mucin and epitheliocytes

^○Zhou Binghui^1,2, Masumizu Yuki^1,2, Nakano Yuhka^1,2, Indoh Yuhki^1,2, Komatsu Ryoya^1,2, Islam Md. Aminul^1,2, Ohtsubo Wakako^1,2,3, Suda Yoshihito⁴, Suzuki Keiichi¹, Aso Hisashi^1,2,3, Kitazawa Haruki^1,2,3 (1.Tohoku Univ. (Agri.), 2.CFAI, 3.JSPS Core-to-Core Program, 4.Miyagi Univ.)

[Objective] Synbiotics, a combination of prebiotics and probiotics, have been considered as potential candidates for antimicrobial substitutes. Wakame, an edible seaweed, has prebiotic properties. To select potential synbiotics, we evaluated adhesion and wakame assimilation ability of eight strains of Lactobacilli isolated from wakame-fed pigs.
[Methods] Mucin-adhesion was evaluated by Biacore assay. Fluorescence was measured after co-incubation of labeled bacteria and PIE cells. PH, turbidity, viable bacterial count and sugar consumption were measured following incubation of Lactobacillus strains in the enzyme-treated wakame medium.
[Results] Three strains showed a higher adhesion ability to sPIM than to sHCM. Only one strain strongly adhered to PIE cells. All strains could assimilate enzyme-treated wakame. Some sugars were consumed by Lactobacilli, indicating that the Lactobacillus strains could utilize the carbon source from Wakame, which often considered as prebiotics.