繁殖・生殖工学

[IV-18-02] Impact of KnockOut™ Serum Replacement in the culture of chicken primordial germ cells

*小出 茅洋¹、濵井 奈津子¹、Effrosyni Fatira²、野村 茂²、竹之内 惇^2,3、都築 政起^1,2,3、中村 隼明^1,2,3 (1. 広島大生物生産、2. 広島大院統合生命、3. 広島大日本鶏資源開発プロジェクト研究セ)

Culture of chicken primordial germ cells (PGCs) together with transfer procedures can facilitate efforts in cryobanking. Chicken PGCs can be proliferated in a defined medium containing key growth factors with the addition of transferrin instead of serum, but its efficiency still remains low. Here, we demonstrated the impact of KnockOut^TM Serum Replacement (KSR), a defined, serum-free formulation, in serum-free culture of chicken PGCs. To investigate the effect of KSR on growth, 1,000 PGCs were cultured with defined medium lacking serum or containing KSR for 10 days. As a result, the addition of KSR enhanced the growth of PGCs. In order to examine whether KSR could improve the derivation efficiency, blood (1μL) containing PGCs was collected from 2-day-old embryos and cultured in a defined medium with either addition or absence of KSR. In all, the addition of KSR accelerated the derivation of PGCs. In conclusion, we demonstrated that the addition of KSR to a defined medium improves the derivation and propagation of chicken PGCs in culture.