繁殖・生殖工学

[IV-18-11] Gene expression of IRFs in bovine endometrial epithelial and stromal cells in vitro

*Qudratullah Qani¹, Al-Nur Md. Iftekhar Rahman¹, Hayato Yamaguchi¹, Daichi Nishino¹, Shutaro Horaku¹, Nobuhiko Yamauchi¹ (1. Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu Univ.)

Interferon regulatory factors (IRFs) have a key role in IFN signaling for ISGs expression in the endometrium of ruminants including bovine. However, bovine endometrial cell types are not characterized for the expression of IRFs. The aim of this study was to characterize bovine endometrial epithelial (BEE) and stromal (BES) cells for the expression of the IRFs in vitro. Both types of cells were cultured without or with E2, P4 and IFNα for 24 h. Gene expression of IRF1, 4, 6 and 9 was analyzed. The result of RT-PCR showed that BEE expressed IRF1 and 6, and BES expressed only IRF1. IRF4 and 9 was not detected in both cell types. However, IRF9 was expressed in both cell types treated with IFNα. RT-qPCR results showed that IFNα significantly increased the expression of IRF9 for BEE, and IRF1 and 9 for BES (P<0.05). There was no difference in the expression of IRF6 in BEE. The present study revealed that IRF expression differs between BEE and BES in vitro. Furthermore, it was shown that IRF1 and 9 are promoted by IFNα, which is type-I IFN as IFNτ. This study provides useful information to elucidate the signaling mechanism of type-I IFN in bovine endometrium.