[V-15-15] Limosilactobacillus ingluviei C37 from broiler inhibits inflammation in LPS-stimulated mouse macrophages
[Objectives] Probiotics are growing alternatives to antibiotics, which can contribute to the prevention and treatment of diseases and enhance livestock production. Previously we isolated Limosilaactobacillus ingluviei C37 (named “LIC37”) from broiler[1]. In this study, we evaluated the immunomodulatory properties of LIC37 to further investigate its probiotic potential.
[Methods] Peritoneal macrophages were isolated from C57BL/6 mice and incubated at 37 °C for two hours in 5% CO2 (2 x 106 cells/well). The cells were treated with heat-killed LIC37 or Lacticaseibacillus rhamnosus GG followed by 2-hour incubation, and stimulated with LPS. TNF-α, IL-6 and IL-10 level was measured by qPCR and ELISA.
[Results] LIC37 strongly suppressed TNF-α and IL-6 level, while promoted IL-10 level in LPS-induced inflammation. These results suggest that LIC37 exerts anti-inflammatory effects by modulating cytokine profiles, and highlight the potential use of this bacterium as an animal probiotic strain.
[1] Tsukagoshi et al., Anim Sci J., 91(1), e13436, 2020.
[Methods] Peritoneal macrophages were isolated from C57BL/6 mice and incubated at 37 °C for two hours in 5% CO2 (2 x 106 cells/well). The cells were treated with heat-killed LIC37 or Lacticaseibacillus rhamnosus GG followed by 2-hour incubation, and stimulated with LPS. TNF-α, IL-6 and IL-10 level was measured by qPCR and ELISA.
[Results] LIC37 strongly suppressed TNF-α and IL-6 level, while promoted IL-10 level in LPS-induced inflammation. These results suggest that LIC37 exerts anti-inflammatory effects by modulating cytokine profiles, and highlight the potential use of this bacterium as an animal probiotic strain.
[1] Tsukagoshi et al., Anim Sci J., 91(1), e13436, 2020.