栄養・飼養Ⅱ

[III-20-06] Effect of orally administered Ligilactobacillus salivarius and its CbpA mutant on TLR3-mediated intestinal immunity

*Sudeb Saha¹, Hikari Yamamuro¹, Yoshiya Imamura¹, Mariano Elean², Leonardo Albarracin ², Binghui Zhou¹, Fu Namai¹, Wakako Ikeda-Ohtsubo¹, Keita Nishiyama¹, Julio Villena², Haruki Kitazawa¹ (1. Food and Feed Immunology Group, Laboratory of Animal Food Function, Graduate School of Agricultural Science, Tohoku University, Sendai 980-8572, Japan, 2. Laboratory of Immunobiotechnology, Reference Centre for Lactobacilli (CERELA-CONICET), Tucuman 4000, Argentina)

[Objectives] Ligilactobacillus salivarius modulate TLR3 mediated innate immune response in porcine intestinal epithelial cells and its surface layer protein choline binding protein A(CbpA) is involved in its ability to adhere and colonize the intestinal epithelim. In this study, we compared the capacity of the wild type (WT) and a CbpA mutant (ΔCbpA) strains to modulate intestinal antiviral immunity in vivo.
[Methods] Infant BALB/c mice (3 weeks-old) were orally treated with WT or ΔCbpA strains for 5 d (10⁸ cells/d/mouse) and then stimulated with poly (I:C) by intraperitoneal injection to induce intestinal inflammation. Non lactobacilli treated mice were used as controls.
[Results] The WT strain reduced the inflammatory mediated intestinal damage and differentially modulated antiviral factors (IFN-β, IFN-γ, Mx1, Mx2, OAS1, OAS2, RNAsel), inflammatory cytokines (TNF-α, IL-6, IL-15) and regulatory cytokines (IL-10, IL-27) in the intestine. The ΔCbpA was not efficient to modulate TLR3 mediated immunity and reduce damage. CbpA is a key factor for L. salivarius immunomodulatory properties.
[Acknowledgement] JSPS P22080, BRAIN 01002AB2