[ODP1A] a. 系統解析・分類・タイピング

[ODP-001] 日本人児童口腔から分離された新菌種Veillonella nakazawaeの提唱

○眞島 いづみ^1,2，シトラ セオドレア³，中澤 太³，アリアドナ デジャイス³，國弘 忠夫⁴，河村 好章²，齊藤 正人⁵，大友 麻衣子⁵，玉井 利代子¹，清浦 有祐¹ (¹奥羽大・歯・口腔病態解析制御・口腔感染免疫，²愛知学院大・薬・微生物，³インドネシア大学・歯・口腔生物，⁴（株）テクノスルガ・ラボ，⁵北海道医療大・歯・小児歯科)

Purpose: Recently, genus Veillonella have received a lot of attention to be a natural probiotic seed, because it was reported that they could utilize lactate in the human bodies for promoting the ability of exercise. Two strains of previously unknown gram-negative cocci belonging to genus Veillonella, T1-7^T and S6-16, were isolated from the oral cavity of healthy Japanese children. In this study, we have established these two strains as a novel Veillonella species based on the genomic, phylogenetic and phenotypic data.
Methods: After sequencing of 4 house keeping genes, phylogenetic analysis were performed. In addition, ANI and dDDH were calculated by using T1-7^T whole genome sequence data. Biochemical characteristics were also analyzed.
Results: Two strains showed the atypical catalase production for genus Veillonella. They are phylogenetically homogeneous and form a distinct, novel lineage within the genus Veillonella shared the highest similarities at 99.9%, 95.8%, 96.9%, and 96.7% using the partial 16S rRNA, dnaK, rpoB, and gltA gene sequences, respectively. Furthermore, strain T1-7^T shared the highest ANI (94.06%) and dDDH value (55.5%) with the most closely related species.
Conclusion: According to these observations, the two strains represent a novel species for which the name Veillonella nakazawae sp. nov. is proposed. The type strain is T1-7^T (JCM 33966^T = CCUG 74597^T).