[ODP3A] a. 菌体表層構造・膜構造・細胞骨格

[ODP-028] Helicobacter pyloriの菌体内輸送ルートとFtsZ分子の関係

○呉 紅¹，高山 省一¹，坂口 翔一¹，岩井 伯隆²，鈴木 陽一¹，中野 隆史¹ (¹大阪医大・予防・社会医学講座・微生物学教室，²東工大・生命理工学院・生命理工学系)

Helicobacter pylori possesses intrabacterial nanotransportation system (ibNoTS) for transporting CagA, VacA, and urease within the bacterial cytoplasm, which is controlled by the extrabacterial environment. The route of ibNoTS for CagA is reported to be associated with the MreB filament, whereas the route of ibNoTS for urease is not yet known. In this study, we demonstratedshowed by immunoelectron microscopy that urease along the route of ibNoTS localizes closely with the FtsZ filament in the bacterium. Supporting this, we found by enzyme immunoassay analysis and co-immunoprecipitation analysis that urease interacts interacted with FtsZ. These findings indicate that urease along the route of ibNoTS is closely associated with the FtsZ filament. Since these phenomena associations were not observed in ibNoTS for CagA, the route of ibNoTS for CagA is considered to be different from that of ibNoTS for urease. We propose that the route of ibNoTS for urease is associated with the FtsZ filament in Helicobacter pylori.