[ODP3A] a. 菌体表層構造・膜構造・細胞骨格

[ODP-029] 臨床分離株Mycobacterium intracellulare Ku11株由来新規糖ペプチド脂質抗原の糖鎖延長遺伝子群解析

○藤原 永年¹，中屋 愼²，宮本 友司³，綾田 稔⁴，中 崇¹，前田 伸司⁵ (¹帝塚山大学現代生活学部食物栄養学科，²大手前栄養学院専門学校，³国立感染症研究所ハンセン病研究センター，⁴大阪市立大学大学院医学研究科ウイルス学，⁵北海道科学大学薬学部薬学科)

MAC (Mycobacterium avium-intracellulare complex) is classifiable into 28 serotypes based on the serotype-specific glycopeptidolipid (GPL) antigen. Mycobacterium intracellulare Ku11 strain was isolated from a patient in Kumamoto. The Ku11 strain produced a novel GPL. The oligosaccharide moiety was defined as α-Rha-(1→3)-2-O-Me-α-Rha-(1→3)-α-Rha-(1→3)-α-Rha-(1→3)-α-Rha-(1→2)-6-d-α-Tal. The Ku11-GPL was composed of 6 pieces of non-acylated sugars. We are now trying to clarify the characterization of the gene cluster involved in GPL biosynthesis. We checked the similarity of the deduced amino acid sequences in the open reading frames (orfs), and tried to introduce orf6, 8, 16 into the same species, M. intracelullare NF113 strain which produce serotype 1-GPL. The sugar moiety of GPL was elongated, and the structure of sugar moiety was identified by ESI/MS/MS. As the results, we identified the orf6, 8, 16 that is functionally responsible for the elongation of oligosaccharide, glycosyltransferase. Now, it is possible to reconstruct 5 pieces of sugar in Ku11-GPL by introduction of orf6, 8, 16 into NF113 strain. We technically established the method of introduction of GPL biosynthesis orfs into M. intracelullare and structural identification by ESI/MS/MS. To clarify the complete biosynthesis genes of the Ku11-GPL, we discuss the structural and functional diversity of GPLs.