[ODP-212] 緑膿菌のAIA-1による抗菌薬抵抗性に関連する遺伝子の同定
Chronic Pseudomonas aeruginosa infection is difficult to eradicate because of antibiotic tolerance, which is the ability of bacteria to survive but not grow in the presence of antibiotics above the minimum inhibitory concentration. We previously demonstrated that the new compound, autoinducer analog-1 (AIA-1) enhanced the antibacterial activity of antibiotics without affecting antibiotic susceptibility of P. aeruginosa, however, the mechanisms of AIA-1 is still unclear. The aim of this study was to investigate the mechanisms of AIA-1 in decreasing antibiotic tolerance. In this study, we constructed the transposon mutagenesis library by mating wild type P. aeruginosa (PAO1) with Escherichia coli S17-1-λ pir harboring pUT-miniTn5pro. Killing assays were performed by using mutants with biapenem or combination of biapenem and AIA-1. Among more than 3700 Transposon mutants, 6 mutants which exhibited high antibiotic tolerance in the presence of AIA-1 and biapenem, and 6 genes were identified by sequencing. Two obtained genes produce hypothetical proteins which functions and pathways yet to be elucidated while four other obtained genes have their own respective functions. Those four are related to metabolism and efflux membrane transporter activity. These genes had critical role in elucidating the mechanisms of AIA-1 in decreasing antibiotic tolerance of P. aeruginosa.