[ODP8A] a. Applications of Microorganisms and Microbial Products

[ODP-229] Bilateral analgesic effect of botulinum toxin type A in chemotherapy-induced peripheral neuropathy

○Yumiko Yamamoto¹, Arief Waskitho², Huijiao Yan², Resmi Raju², Swarna Lakshmi Raman², Takehiko Mima¹, Kazuyoshi Gotoh¹, Kenji Yokota³, Osamu Matsushita¹, Yoshizo Matsuka² (¹Dept. Bacteriol., Grad. Sch. Med. Dent. Pharm. Sci., Okayama Univ., ²Grad. Sch. Biomed. Sci., Tokushima Univ., ³Grad. Sch. Health Sci., Okayama Univ.)

We have previously shown that botulinum toxin type A (BoNT/A) alleviates trigeminal neuropathy induced by infraorbital nerve constriction. In present study, we generated two rat models of chemotherapy-induced peripheral neuropathy, and assessed the analgesic effect of BoNT/A in chemotherapy-induced neuropathy. Sprague-Dawley male rats (120-140 g) were intraperitoneally injected with anticancer medication, cisplatin (2 mg/kg/day) once a day for four days or vincristine sulfate (100 µg/kg/day) in two 5-day cycles with two days break between cycles (10 injections, 1 mg/kg cumulative dose). Administration of cisplatin or vincristine sulfate produced bilateral mechanical allodynia which was measured as decreased head withdrawal threshold (p < 0.05). BoNT/A (10 MLD) or saline was intracutaneously administered on one side at the center of the whisker pad 15 days after the first administration of the anticancer medication. Unilateral BoNT/A administration in the whisker pad area attenuated chemotherapy-induced mechanical allodynia bilaterally. This result suggests that BoNT/A has the potential central effect.