[ODP31] 7. Antimicrobial agents and resistance -b. Antimicrobial resistance

[ODP-217] Characteristics of E. coli isolated from urinary tract infections at the Kyorin University Hospital

Nhat Minh Le¹, Tomoya Suda², Mayuko Tanaka³, Shota Yonetani⁴, Takeaki Matsuda^1,2, Tomoko Hanawa³ (¹Dept. Traumatology and Critical Care Medicine, Kyorin Univ. Sch. Med., ²Dept. General Medicine, Kyorin Univ. Sch. Med., ³Dept. Infectious Diseases, Kyorin Univ. Sch. Med., ⁴Dept. Laboratory Medicine, Kyorin Univ. Sch. Med.)

Background & Objective: E. coli is the major causative agent (80%) of urinary tract infections (UTIs). The ESBL-EC strains causing UTIs which held account for 15.5% of hospitalizations and 6.2% of death in adults of 65 years. To develop phage therapy targeted to the ESBL-EC causing UTIs, we firstly investigated the characteristics of those strains.
Materials & Methods: Sensitivities against antibiotics were examined according to the CLSI guidelines. Sequence type (ST) and ESBL CTX-M genes, phylogenetic-group, and virulence factor were detected by Cica Genus E. coli POT Kit and multiplex PCR.
Results & Discussion: A total of 55 E. coli strains that were resistant (100%) to ABPC, PIPC, CAZ, CEZ, CEPM, CTM, CPDX-PR, CXM, AZT, following to SBT/ABPC (84%), CPFX (80%), LVFX (80%), CFDN (76%), ST (34%), GM (28%) and MINO (4%), but susceptible (100%) to CMZ, LMOX, TAZ/PIPC and AMK. Phylogenetic-group B2 and D were 48 (87%) and 7(13%). The prevalence of sequence type (ST)131, CTX-M-9 and CTX-M-1 were 45 (82%), 37 (67%), and 12 (22%). The frequency of the fimH, fyuA, ompT, iutA, kpsMTII, usp, traT, papGII, papAH, hlyA and cnf1 were 55 (100%), 54 (98%), 48 (87%), 47 (85%), 39 (71%), 34 (62%), 26 (47%), 13 (24%), 12 (22%), 12 (22%), and 9 (16%). In summary, phage therapy targeted to ESBL-EC strains with predominate of morphology B2-ST131-CTX-M-9 causing UTIs is more effective for treatment.