Session 10

[BISC10-02(Invited)] Multi-photon Microscopy Enhanced by Manipulation of Excitation Laser Beam and its Application to Cellular Physiology

*Tomomi Nemoto^1,2,3,4, Kohei Otomo^1,2,5, Hirokazu Ishii^1,2,3, Motosuke Tsutsumi² (1. Exploratory Research Center on Life and Living Systems (ExCELLS), Nat. Inst. Nat. Sci., 2. Nat. Ins. Physiol. Sci., Nat. Inst. Nat. Sci., 3. Grad. Univ. Adv. Studies (SOKENDAI), 4. Res. Inst. Electro. Sci., Hokkaido Univ., 5. Grad. Sch. Med., Juntendo Univ.)

Multi-photon excitation fluorescence microscopy is a robust technique for understanding physiological phenomena from the cellular to the tissue level.By taking advantage of novel laser light technologies, we have successfully improved fluorescent signal-to-noise ratio and temporal and spatial resolutions while observing physiological events in living cells and animals.