Session 10

[BISC10-04] Volume Hologram-based Airy Light-sheet Microscopy

*Hung-Chuan Hsu¹, Sunil Vyas², Kuang-Yuh Huang¹, Hsien-Shun Liao¹, Yuan Luo^2,3,4,5 (1. Department of Mechanical Engineering, National Taiwan University, 2. Institute of Medical Devices and Imaging System, National Taiwan University, 3. YongLin Institute of Health, National Taiwan University, 4. Department of Biomedical Engineering, National Taiwan University, 5. Graduate School of Advanced Technology, National Taiwan University)

Light-sheet fluorescence microscopy (LSFM) offers rapid optical sectioning with low photobleaching and phototoxicity. Combining LSFM with Airy beams via volume holograms enhances the field of view and image quality, presenting advantages like expanded field of view, increased penetration depth, and improved imaging quality in measuring large biological samples.