[1P-0721] Highly efficient CRISPR/Cas9-mediated knock-in by electroporation using long ssODN in zygote

〇Sei Kondo¹, Miho Kuwahara¹, Shanqi Fu¹, Kenji Ikeda², Takanori Ishikawa¹, Mitsuaki Ono³, Takashi Nishida¹, Satoshi Kubota¹, Takako Hattori¹ (1.Dept of Biochem Mol Dent, Okayama Univ Grad Sch of Med, Dent Pharm Sci, 2.Okayama University Dental School, 3.Dept of Mol Biol Biochem,Okayama Univ Grad Sch of Med, Dent Pharm Sci)

The 41st Annual Meeting of the Molecular Biology Society of Japan

[1P-0722] The establishment of knock-in, knock-out, and conditional knock-out mice using Crispr/Cas9 system by fertilized egg injection.

〇Riki Ishibashi^1,2, Kota Abe^1,2, Fumiko Toyoshima^1,2 (1.Dept. of Bios. Sci., Lab. of Tissue Homeostasis, Inst. for Front. and Med. Sci., Kyoto Univ., 2.Dept. of Mammal. Regu. Net., Lab. of Cell Divi. and Diff., Grad. Sch. of Biost. Kyoto Univ.)

The 41st Annual Meeting of the Molecular Biology Society of Japan

[1P-0723] Large fragment knock-in by zygote genome editing with adeno-associated viral vector and CRISPR/Cas9.

〇Naoaki Mizuno¹, Eiji Mizutani¹, Hideyuki Sato¹, Mariko Kasai¹, Tomoyuki Yamaguchi¹, Hiromitsu Nakauchi^1,2 (1.Div. of Stem Cell Therapy, IMSUT, University of Tokyo., 2.Inst for Stem Cell Biol and Reg Med, Stanford University.)

The 41st Annual Meeting of the Molecular Biology Society of Japan

[1P-0724] Genome editing technology expands the potential of rat resources

〇Kosuke Hattori¹, Yoshiki Miyasaka¹, Yuko Yamauchi², Kanako Shimizu¹, Yoshihiro uno¹, Kazuto Yoshimi², Tomoji Mashimo^1,2 (1.IEXAS,Grad.sch.of med.Osaka Univ., 2.GERD Center,Grad.sch.of med.Osaka Univ.)

The 41st Annual Meeting of the Molecular Biology Society of Japan

[1P-0725] Attempt to generate an improved immunodeficient model marmoset using genome editing technology

〇KENYA SATO¹, TOMOE MORIOKA¹, WAKAKO KUMITA¹, TETSUSHI SAKUMA², EIKO YAMAZAKI¹, YOKO KUROTAKI¹, TAKASHI YAMAMOTO², ERIKA SASAKI¹ (1.Dept. of marmoset research, Central Institute for Experimental Animals, 2.Grad. Sch. Sci., Univ. Hiroshima)

The 41st Annual Meeting of the Molecular Biology Society of Japan

[1P-0726] Engineering of IgG-like trispecific T cell-recruiting antibodies by domain exchange

〇Ryota Nakamura¹, Chihiro Tsumura², Masaya Kitamura¹, Takeshi Nakanishi¹ (1.Dept. of Appl. Chem. and Bioeng., Grad. Sch. of Eng., Osaka City Univ., 2.所属なし)

The 41st Annual Meeting of the Molecular Biology Society of Japan

[1P-0727] Improving the affinity of an antibody by introducing charged amino acids into framework region 3

〇Fukunaga Atsushi¹, Reema bajaj¹ (1.sysmex corporation)

The 41st Annual Meeting of the Molecular Biology Society of Japan

[1P-0728] Development of small antibody mimetics with high-affinity and specificity for imaging and targeting HER2-positive tumors using structurally constrained CDR peptides

〇Wanaporn Yimchuen¹, Tetsuya Kadonosono¹, Shinichi Sato², Hiroyuki Nakamura², Kyra See¹, Takahiro Kuchimaru³, Shinae Kondoh¹ (1.School of Life Science and Technology, Tokyo Institute of Technology, 2.Institute of Innovative Research, Tokyo Institute of Technology, 3.Center for Molecular Medicine, Jichi Medical University)

The 41st Annual Meeting of the Molecular Biology Society of Japan

[1P-0729] Development of platform for generating antibody alternatives from CDR peptides

〇Marina Katsumi¹, Kyra See¹, Tetsuya Kadonosono¹, Takahiro Kuchimaru², Shinae Kondoh¹ (1.School of Life Science and Technology, Tokyo Institute of Technology, 2.Center for Molecular Medicine, Jichi Medical University)

The 41st Annual Meeting of the Molecular Biology Society of Japan

[1P-0730] Development of high affinity HER2-targeting small protein using fibronectin type III as a scaffold

〇Yumi Ota¹, Tetsuya Kadonosono¹, Takahiro Kuchimaru², Masumi Taki³, Yuji Ito⁴, Shinae Kondoh¹ (1.School of Life Science and Technology, Tokyo Institute of Technology, 2.Center for Molecular Medicine, Jichi Medical University, 3.Graduate School of Informatics and Engineering, the University of Electro-Communications, 4.Graduate School of Science and Engineering, Kagoshima University)

The 41st Annual Meeting of the Molecular Biology Society of Japan