[1P-0711] Simple and efficient induction of skeletal muscle cell from hiPSCs by using Tet-MyoD inducible piggyBac vector

〇Jun Otomo¹, Michiko Nakamura¹, Knut Woltjen¹, Sakurai Hidetoshi¹ (1.Kyoto University iPS cell research center)

The 41st Annual Meeting of the Molecular Biology Society of Japan

[1P-0712] Genome-wide genetic screening of osteoclastogenesis-related genes with a lentiviral CRISPR-guide RNA library

〇Yuki Nakano¹, Yuu Taguchi¹, Jun-ichiro Inoue¹ (1.Division of Cellular and Molecular Biology, The Institute of Medical Science, The University of Tokyo)

The 41st Annual Meeting of the Molecular Biology Society of Japan

[1P-0713] Developing a microRNA-responsive CRISPR-Cas9 ON/OFF system to conduct cell-specific genome-editing

〇Moe Hirosawa^1,2, Yoshihiko Fujita², Hirohide Saito² (1.Grad Sch of Med, Kyoto Univ, 2.CiRA, Kyoto Univ)

The 41st Annual Meeting of the Molecular Biology Society of Japan

[1P-0714] Genome-wide screening for off-target mutations in vivo using CIRCLE-seq

〇Masayuki Tanaka¹, Keiko Yokoyama¹, Hideki Hayashi¹, Sanae Isaki¹, Kanae Kitatani¹, Ting Wang¹, Hisako Kawata¹, Hideyuki Matsuzawa¹, Hiromi Miura^2,4, Susumu Takekoshi^3,1, Masato Ohtsuka² (1.Support Center for Medical Research and Education, Tokai University, 2.Department of Molecular Life Science, Tokai University School of Medicine, 3.Department of Cell Biology, Tokai University School of Medicine, 4.Department of Regenerative Medecine, Tokai University School of Medicine)

The 41st Annual Meeting of the Molecular Biology Society of Japan

[1P-0715] CRISPR-Cas9 RNP delivery via virus-like particles reduces off-target editing

〇Takahiro Iguchi^1,3, Peter Gee^1,2, Akitsu Hotta^1,2 (1.CiRA, Kyoto Univ., 2.iCEMS, Kyoto Univ., 3.Faculty of Agric., Kyoto Univ.)

The 41st Annual Meeting of the Molecular Biology Society of Japan

[1P-0716] Genome editing technology-mediated generation of NBS1 I171V variant knock-in mice

〇Tatsuo Miyamoto¹, Keita Tomioka¹, Silvia Natsuko Akutsu¹, Takashi Yamamoto², Hiroshi Tauchi³, Shinya Matsuura¹ (1.Dept of Genet and Cell Biol, RIRBM, Hiroshima Univ, 2.Dept Math and Life Sci, Grad Sch Sci, Hiroshima Univ, 3.Dept of Biol Sci, Fac Sci, Ibaraki Univ)

The 41st Annual Meeting of the Molecular Biology Society of Japan

[1P-0717] Durable transgene expression from \"usual\" plasmids in mouse liver

〇Hiroyuki Kamiya^1,2, Yusuke Wakao², Tetsuya Suzuki^1,2 (1.Grad. Sch. Biomed. Hlth. Sci., Hiroshima Univ., 2.Sch. Pharm. Sci., Hiroshima Univ.)

The 41st Annual Meeting of the Molecular Biology Society of Japan

[1P-0718] miRNA-sensitive synthetic mRNA responds to endogenous target miRNA in vitro and in vivo

〇Shunsuke Wada¹, Kaoru Richard Komatsu¹, Yi Kuang¹, Hirohide Saito¹ (1.Center for iPS cell Research and Application, Kyoto University)

The 41st Annual Meeting of the Molecular Biology Society of Japan

[1P-0719] Efficient generation of conditional knockout mice by the 2-step method

〇Takuro Horii¹, Ryosuke Kobayashi¹, Eriko Suetomo¹, Yuika Kawada¹, Mika Kimura¹, Sumiyo Morita¹, Izuho Hatada¹ (1.Biosignal Genome Resource Center, IMCR, Gunma University)

The 41st Annual Meeting of the Molecular Biology Society of Japan

[1P-0720] Generation of genome editing mice using cryopreserved embryos and electroporation

〇Yuko Kotani¹, Arisa Tanigawa¹, Kanako Shimizu¹, Yoshihiro Uno¹, Kazuto Yoshimi^1,2, Tomoji Mashimo^1,2 (1.Inst of Exp Anim Sci, Univ of Osaka, 2.Geno Edit R&D Center,Univ of Osaka)

The 41st Annual Meeting of the Molecular Biology Society of Japan