[3P-0659] Evaluating stability dependence of enzyme mutability through combinatorial suppression of amber mutations.

^○Shunta Takenaka¹, Masakazu Araki¹, Soji Watanabe¹, Kensaku Sakamoto², Shigeko Kawai(Noma)¹, Daisuke Umeno^{1, 3} (1.Grad. Sch. Of Eng., Chiba Univ., 2.RIKEN, Center for Life Sci. Technol., 3.Grad. Sch. Of Sci. and Eng., Waseda Univ.)

The 44th Annual Meeting of the Molecular Biology Society of Japan

[3P-0660] A construction of useful binding assay for interactions PDZ domain and carboxy-terminal tail by using microtiter plates

^○Takeshi Tenno, Haruka Takagi, Uiko Tomiyasu, Hidekazu Hiroaki (Grad. Sch. Pharma. Sci., Nagoya Univ.)

The 44th Annual Meeting of the Molecular Biology Society of Japan

[3P-0661] Simple and efficient genetic engineering of enteroids by using mouse isolated crypts

^○Shuya Ohira¹, Yuki Yokoi^{1, 2}, Mani Kikuchi², Natsumi Yatsuzuka¹, Tokiyoshi Ayabe^{1, 2}, Kiminori Nakamura^{1, 2} (1.Innate Immunity Lab., Grad. Sch. of Life Sci., Hokkaido Univ., 2.Innate Immunity Lab., Dept. of Cell Biol. Sci., Faculty of Adv. Life Sci., Hokkaido Univ.)

The 44th Annual Meeting of the Molecular Biology Society of Japan

[3P-0662] Application of chromosome engineering technology (1): Construction of a panel of human cell lines containing human/mouse artificial chromosomes for the dissemination of chromosome engineering technology

^○Narumi Uno¹, Hitomaru Miyamoto², Shuta Takata³, Shinya Komoto², Ryota Mayuzumi², Natsumi Miyazaki¹, Yuji Nakayama⁴, Mitsuhiko Osaki⁵, Chiaki Hando¹, Satoshi Abe³, Tetsushi Sakuma⁶, Takashi Yamamoto⁶, Teruhiko Suzuki⁷, Yoshihiro Nakajima⁸, Mitsuo Oshimura³, Kazuma Tomizuka¹, Yasuhiro Kazuki^{2, 3} (1.Lab. of Bioeng., Sch. of Life Sci., Tokyo Univ. of Pharm and Life Sci., 2.Div. of Genome and Cell. Func., Grad. Sch. of Med. Sci., Tottori Univ., 3.CERC, Tottori Univ., 4.Div. of Radioiso. Sci., Org. for Res. Initi. and Prom., Tottori Univ., 5.Div. of Exp. Pathol., Facul. of Med., Tottori Univ., 6.Div. of Integrat. Sci. for Life, Grad. Sch. of Integrat. Sci. for Life, Hiroshima Univ., 7.Stem Cell Proj., Tokyo Metropol. Inst. Med. Sci., 8.KISTEC)

The 44th Annual Meeting of the Molecular Biology Society of Japan

[3P-0663] Application of chromosome engineering technology (2): Understanding the genome regulation of mouse Y chromosome by synthetic biology

^○Kyotaro Yamazaki¹, Kyosuke Matsuo¹, Chihiro Yoshimatsu¹, Naoyo Kajitani², Miho Yamakawa³, Kanako Kazuki³, Yasuhiro Kazuki^{1, 3} (1.Div. of Genome and Cell. Func., Grad. Sch. of Med. Sci., Tottori Univ., 2.Interdisciplinary Cen., Shimane Univ., 3.CERC, Tottori Univ.)

The 44th Annual Meeting of the Molecular Biology Society of Japan

[3P-0664] Application of chromosome engineering technology (3): Generation of HLA-cluster model mouse using mouse artificial chromosome vector

^○Nanami Kishima¹, Takashi Moriwaki^{1, 2}, Kanako Kazuki², Wakana Nakagawa³, Narumi Uno⁴, Kazuma Tomizuka⁴, Teruhiko Suzuki⁵, Yasuhiro Kazuki^{1, 2} (1.Div. of Genome and Cell. Func., Grad. Sch. of Med. Sci., Tottori Univ, 2.CERC, Tottori Univ, 3.Sch. of Life Sci., Facul. of Med., Tottori Univ, 4.Lab. of Bioeng., Sch. of Life Sci., Tokyo Univ. of Pharm. and Life Sci, 5.Stem Cell Proj., Tokyo Metropol. Inst. Med. Sci)

The 44th Annual Meeting of the Molecular Biology Society of Japan

[3P-0665] Application of chromosome engineering technology (4): Development of a direct chromosome transfer method from human iPS cells to animal ES cells for rapid transchromosomic animal production

^○Chihiro Yoshimatsu¹, Hitomaru Miyamoto¹, Kyotaro Yamazaki¹, Narumi Uno², Teruhiko Suzuki³, Kanako Kazuki⁴, Kazuma Tomizuka², Yasuhiro Kazuki^{1, 4} (1.Div. of Genome and Cell. Func., Grad. Sch. of Med. Sci., Tottori Univ., 2.Lab. of Bioeng., Sch. o Life Sci., Tokyo Univ. of Pharm. and Life Sci., 3.Stem Cell Proj., Tokyo Metropol. Inst. Med. Sci., 4.CERC, Tottori Univ.)

The 44th Annual Meeting of the Molecular Biology Society of Japan

[3P-0666] Application of chromosome engineering technology (5): Analysis of brain function in a rat model of Down syndrome

^○Risa Matsumoto¹, Masaharu Hiratsuka², Yasuhiro Kazuki^{2, 3} (1.Div. of Genome and Cell. Func., Grad. Sch. of Med. Sci., Tottori Univ., 2.Sch. of Life Sci., Facul. of Med., Tottori Univ., 3.CERC, Tottori Univ.)

The 44th Annual Meeting of the Molecular Biology Society of Japan

[3P-0667] Application of chromosome engineering technology (6): Establishment of an in vitro cell model to evaluate TDP-43 proteinopathy

^○Shota Togai¹, Masaharu Hiratsuka², Yasuhiro Kazuki^{1, 3} (1.Div. of Genome and Cell. Func., Grad. Sch. of Med. Sci., Tottori Univ., 2.Sch. of Life Sci., Facul. of Med., Tottori Univ., 3.CERC, Tottori Univ)

The 44th Annual Meeting of the Molecular Biology Society of Japan

[3P-0668] Application of chromosome engineering technology (7): Analysis of allelic exclusion in fully human antibody producing mice

^○Takashi Moriwaki¹, Hiroyuki Satofuka², Satoshi Abe³, Hiroshi Tanaka³, Kayoko Morimoto³, Kazuma Tomizuka⁴, Mitsuo Oshimura³, Yoshihiro Baba⁵, Yasuhiro Kazuki^{1, 2} (1.Sch. of Life Sci., Facul. of Med., Tottori Univ., 2.CERC, Tottori Univ., 3.Trans Chromosomics Inc., 4.Lab. of Bioeng., Sch. of Life Sci., Tokyo Univ. of Pharm. and Life Sci., 5.Div. Immunol. and Genome Biol., Depart. of Mol. Genet., Med. Inst. of Bioreg., Kyushu Univ.)

The 44th Annual Meeting of the Molecular Biology Society of Japan