[P2-102] Antioxidative Scavenger Potency of Nitric Oxide or Glutathione in Children with Epilepsy or in Epilepsy-Prone EL Mice
[Introduction] It is well known that oxidative stress is closely related to epiletogenesis. It has been reported that oxidative stress is upregulated in the central nervous system (CNS) compartment in patients with epilepsy, so it is supposed that the levels of endogenous antioxidative scavengers in the cerebrospinal fluid (CSF), which exert some stress on the human body, are elevated as an adaptive reaction to epilepsy. We assumed that nitric oxide (NO), which has potent antioxidative activity was elevated in patients with epilepsy. NO and some other endogenous antioxidative parameters were investigated in the CSF from people with epilepsy or in homogenize of the brain tissues in epilepsy-prone EL mice.
[Methodology] CSF samples in the human were collected from the patients in our hospital with clinical indication of lumber puncture. Homogenize of the brain tissues in EL mice was also collected, with ddY mice inbred from a maternal, nonepileptic strain of EL serving as controls. The study protocol was approved by the Ethic Committee and the Laboratory Animals Committee of Nippon Medical School.
[Results] NO, reduced glutathione (GSH), and glutathione peroxidase (GPX) activities in CSF from people with epilepsy were significantly higher than in those with aseptic meningitis or with the controls. And NO, oxidized glutathione (GSSG), GPX activities were higher in early age of EL mice.
[Conclusions] This study shows that upregulation of NO or GSH-mediated scavenger potency may be related to the acquisition of epileptogenesis in both the human and EL mice.
[Methodology] CSF samples in the human were collected from the patients in our hospital with clinical indication of lumber puncture. Homogenize of the brain tissues in EL mice was also collected, with ddY mice inbred from a maternal, nonepileptic strain of EL serving as controls. The study protocol was approved by the Ethic Committee and the Laboratory Animals Committee of Nippon Medical School.
[Results] NO, reduced glutathione (GSH), and glutathione peroxidase (GPX) activities in CSF from people with epilepsy were significantly higher than in those with aseptic meningitis or with the controls. And NO, oxidized glutathione (GSSG), GPX activities were higher in early age of EL mice.
[Conclusions] This study shows that upregulation of NO or GSH-mediated scavenger potency may be related to the acquisition of epileptogenesis in both the human and EL mice.