[PS02-36] Preliminary report of mating behavior and sex pheromone of Heterolocha aristonaria (Lepidoptera: Geometridae)
Heterolocha aristonaria (Lepidoptera: Geometridae) is a widely distributed species throughout East Asia including Japan, China, and Korea. The larva feeds on Honeysuckle family leaves like Lonicera japonica. H. aristonaria females started calling on the 2nd day after emergence, and the maximum number of calls was observed on the 3rd day. The beginning of calls started at 9 hr after the start of the scotophase with a peak in 10 hr. On GC-EAD analysis, male antennae showed a weak and a strong peak against the crude extract of female sex pheromone. However, no compounds related to the two antennal responses were found in the following GC/MS analysis.To identify those two EAD-active compounds, the crude extract was fractionated on a florisil column, and then laboratory bioassay and GC-EAD analysis were performed on each fraction.Males were well attracted to the 0.3 female equivalent (FE) of crude extract in a wind tunnel, so we gave males a mixture of 0.3 FE of each fraction. When the Hx:EtOAc = 100:0 fraction was removed, the males' responses were significantly reduced. In addition, in GC-EAD analysis, male antennae showed a reproducible response only to the Hx:EtOAc = 100:0 fraction, which was in good agreement with that of the crude extract.