[O12] Concepts, Prospects, and Potentiality of Crop Production in East Asia

[O12-06] Physiological and Proteome Analysis in Brassica napus L. of Leaves in Response to Copper Stress and Citric-Acid Application

*Nominated for Presentation Awards

^○Yong-Hwan Ju¹, Ju Young Choi¹, Swapan Kumar Roy¹, Soo Jeong Kwon¹, Kwang Soo Kim², Sun Hee Woo¹ (1.Dept of Crop Science, Chungbuk National University, Korea, 2.Bio-Energy Plant Research Center, National Institute of Crop Science, Korea)

Copper (Cu) is one of the essential nutrients but excess Cu induces phytotoxicity, leading to inhibit plant growth characteristics. This study aimed to invest the proteome changes of Brassica napus L. under Cu stress and Citric-acid (CA) application over 7 days. The 21-days-old seedlings were exposed to different concentrations of Cu (25 and 50µM CuSO₄) and CA (1.0 mM). Results showed that the fresh and dry weights of Cu + CA treated seedlings were higher than those of the Cu treated seedlings. The application of CA further enhanced the activities of antioxidant enzymes as compared with their respective Cu-only treatment. Using the gel-free proteome approach, a total of 1473 proteins were successfully identified in B. napus seedlings. Of these, 67 proteins were identified only in Brassica species, whereas 36 proteins identified from B. napus that were classified as differentially abundant proteins (DAPs). Among the 36 proteins, a total of 21 proteins were upregulated and 15 proteins were downregulated Cu stress and CA application treated. To understand molecular functions and biological processes involved in B. napus response to Cu stress, Gene Ontology analysis was performed whereas the most of the DAPs were annotated to protein metabolism, fatty acid biosynthetic process, TCA cycle, carbon fixation, photorespiration, fructose catabolic process and glyoxylate cycle. Taken together, this study may provide new insights into the molecular mechanisms of plant response to Cu stress, and CA application may alleviate the stress symptoms.