[MS4-2D-2] Impairment of prenatal exposure to MRI on rats’ spatial memory are associated with CamK IIβ
To investigate the effects of MR prenatal exposure on the ability of learning and memory, we examined the behavior changes of rats after MR prenatal exposure and detect the molecular mechanism of behavior deficit. Fourteen pregnant Sprague–Dawley rats were randomly divided into an experimental group and a control group, each with 7 mother rats. The experimental animals were exposed to MR between days 12 and 18 of gestation for 10 min a day. After birth, all male litters were randomly divided into an MRI-scanning group (experimental group) and a control group. At 1st-, 2nd- and 5th-month after birth, Morris Water Maze (MWM) tasks were tested. The results showed that at the age of 2 month, the MR exposed rats s spent less time than the non-exposed ones in the platform quadrant during probe trial test. Then we selected the 2 months groups for micro-array and Western blot to detect the molecular mechanism of behavior deficit. Here we found that only 12 genes among all the tested were up or down-regulated. Western bolt result showed that CaMKIIβ was changed significantly. We suggested that the potential impairment of spatial learning and memory induced by prenatal MR exposure was age dependence, which may mediated by regulation of CaMKIIβ.