[P2-91] Effect of fasudil hydrochloride on cognitive function after status convulsion in SD rats
Background and objective： Recent studies have reported that HF has a protective effect on neural injury. However, its protective effect on convulsive brain injury has not yet been assessed. Therefore, we explored latent mechanism and effect of HF on cognitive function after SC in rats. Methods: Initially, 26 rats selected were evenly divided into control group and 21dSC1d group to determine the effects of SC on the protein expression levels of Lingo-1 MAG, MOG, NogoA, RhoA and p-RhoA by immunofluorescence and western-blot. Then another 40 rats were evenly divided into group I (normal rats), group II (normal rats treated with HF), group III (SC rats), group IV (SC rats treated with HF), which were selected to explore the cognitive function improvement effects of HF on SC rats by Object-in-place memory task and Morris Water Maze test. One rat from each group of I-IV was chosen to perform HE staining and Nissl staining. After then, SC was induced again to determine the effects of HF on SC latency in the rest rats of group I-IV. Results： After SC, levels of MAG, MOG and Lingo-1 were significantly increased (P< 0.05) and level of NogoA was significantly decreased in rats (P< 0.01). SC had no effect on RhoA level (P=0.921), but significantly promoted p-RhoA level (P< 0.01 ). Cognitive function was significantly decreased after SC and significantly promoted after HF intervention. HF intervention promoted CA1 function, which was barely lost after SC. Conclusion： SC seriously impaired cognitive function and affects the expression of neurite growth inhibitory factors. Not only could HF improve cognitive function and CNS injury of rats, but also decrease SC susceptibility. HF and SC may regulate CNS by affecting the expression of neurite growth inhibitory factor in RhoA/ROCK signaling pathway.