[P2-1~135] Poster Presentation 2

[P2-92] Influence of epileptiform discharge microenvironment on the differentiation of oligodendrocyte precursor cells

^○Li Jiang^{1, 2} (1.Lab of Pediatric Neurology, Ministry of Education, Key Laboratory of Child Development and Disorders, Key Laboratory of Pediatrics in Chongqing, Chongqing International Science and Technology Cooperation Center for Child Development and Disorders, Children’s Hospital of Chongqing Medical University, Chongqing, PR China, 2.Department of Neurology, Children’s Hospital of Chongqing Medical University, Chongqing, PR China)

Using related cell cultured model to investigate the genesis and development of epilepsy，is benefit for exploring the mechanism of epileptogenesis. Recent reports have described the damage to myelination of epilepsy in animal models, but few reports focused on the influence of epilepsy on the remyelination in vitro. The current study was designed to the effect of epileptiform discharge neurons microenvironment on the differentiation of oligodendrocyte precursor cells (OPCs). Neurons expressing epileptiform discharge released more glutamic acid than normal neurons detected by HPLC. QT-qPCR, immunofluorescence and Western Blot results showed the MBP loss in the epileptiform discharge neurons microenvironment, and accompanied with GluR2 subunits increasing. In addition, AMPA receptor antagonist could alter the influence of epilepsy on OPCs differentiation. This study confirmed positive effect of epilepsy on OPCs differentiation, and the role of glutamic acid and AMPA receptor in this process, and provided a novel treatment strategy for epilepsy.
Keywords: Oligodendrocyte precursor cell, differentiation, epilepsy, microenvironment, AMPA receptor, GluR2 subnit