[P2-1~135] Poster Presentation 2

[P2-94] Characteristics of spiral wave distribution in neocortex in absence epileptic rats

^○Jianmin Liang^{1, 2, 3} (1.Department of Pediatric Neurology, first hospital of Jilin University, Changchun, China., 2.Research center of Neuroscience, first hospital of Jilin University, Changchun, China., 3.Research center of Pediatrics, first hospital of Jilin University, Changchun, China)

Objective：To explore the pathogenesis of absence epilepsy through the analysis the characteristics of spiral waves distribution in neocortex. Methods：Absence epileptic rat model was established via GBL (150 mg/kg) intraperitoneal injected in rats. Voltage sensitive dying (VSD) imaging was performed combinding with VSD EEG and ECG recording simultaneouly. The distribution characteristics of spiral waves were analyzed in different stages divided depending on the spike slow wave discharges, including background stage (BS), discharge coming stage (DCS), during discharge stage (DDS), discharge ending stage and interictal stage (IS). Results：Absence epileptic rat model was successfully established with EEG clearly present typical spike slow wave discharge regularly. Both of the frequency and duration of spiral waves were significant increased in DES period than that of other stages (BS, DCS, DDS, IS) (P＜0.05). Conclusions：Spiral waves prefer to occur in DES period, suggesting that it may be an important mechanism underlying self-termination of absence seizure in cortex. Therefore, spiral waves may be an important functional regulating pattern for population neurons to control the duration of absence seizures on the fluid dynamics horizon in neocortex.
Acknowledgments:
This work was supported by the National Nature and Science Foundation of China (No. 81171220) and (No. 31371125), the Fund of Pediatric Development from the First Hospital of Jilin University, and the Nature and Science Foundation (No. 20160101123JC) from the Science and Technology Department of Jilin Province.