[P2-96] Fasudil hydrochloride can improve the cognitive dysfunction caused by status convulsion
[Background] Status convulsion (SC) is the most common neurological emergency which may result in bad outcomes with epilepsy or cognitive impairment. Fasudil hydrochloride (FH), an active metabolite of fasudil is a typical Rho kinase (ROCK) inhibitor and it has been reported to improve the cognitive deficits in aged rats and in rats with cerebral ischemia. However, it is not clear whether FH can improve the cognitive deficits of patients with seizure-induced brain injury after SC. So we aimed at discussing whether FH can improve the rats’ cognitive deficits induced by SC.
[Methods] Male young Sprague-Dawley rats (21 days postnatal ) were obtained from the experimental animal center of Chongqing medical university. We randomly divided these rats into 4 groups (n=10 in each group): a, the control; b, SC model without interference; c, FH administration by intra-cerebroventricular injection at the first day (acute phase for developing epilepsy) after SC; d, FH administration by intra-cerebroventricular injection at the fifth day (incubation phase for developing epilepsy) after SC. Rats in the b, c and d groups were induced SC by lithium-Pilocarpine intraperitoneally, then rats in c and d groups were respectively administrated FH at the first or fifth days after SC. The cognitive functions were tested by Morris water maze after 20 days of SC (early chronic phase for developing epilepsy).
[Results] Compared with the control group (group a), in the Morris water maze test, the SC group’s (group b) escaping latency period were significantly longer; the time in the target quadrant were longer in probe trail, cross-platform times were decreased. The group c and d, to some degree, showed positive improvements in escaping latency period, the time in the target quadrant, and cross-platform times. While the group d presented a more remarkable improvement compared with the group b during Morris water maze test.
[Conclusion] The results suggested rats with SC may develop cognitive dysfunction because of seizure- induced brain injury. FH could improve learning and memory abilities of these rats with SC.
[Methods] Male young Sprague-Dawley rats (21 days postnatal ) were obtained from the experimental animal center of Chongqing medical university. We randomly divided these rats into 4 groups (n=10 in each group): a, the control; b, SC model without interference; c, FH administration by intra-cerebroventricular injection at the first day (acute phase for developing epilepsy) after SC; d, FH administration by intra-cerebroventricular injection at the fifth day (incubation phase for developing epilepsy) after SC. Rats in the b, c and d groups were induced SC by lithium-Pilocarpine intraperitoneally, then rats in c and d groups were respectively administrated FH at the first or fifth days after SC. The cognitive functions were tested by Morris water maze after 20 days of SC (early chronic phase for developing epilepsy).
[Results] Compared with the control group (group a), in the Morris water maze test, the SC group’s (group b) escaping latency period were significantly longer; the time in the target quadrant were longer in probe trail, cross-platform times were decreased. The group c and d, to some degree, showed positive improvements in escaping latency period, the time in the target quadrant, and cross-platform times. While the group d presented a more remarkable improvement compared with the group b during Morris water maze test.
[Conclusion] The results suggested rats with SC may develop cognitive dysfunction because of seizure- induced brain injury. FH could improve learning and memory abilities of these rats with SC.