[P3-204] The optimal laser irradiation condition for satellite cell proliferation varies between fast and slow twitch muscles
[Introduction]
Satellite cell (SC), known as the stem cell of skeletal muscle plays the key role in postnatal muscle regeneration. Clinically, the etiology of most progressive muscular atrophy (PMA) are known to be related to the depletion of SC. Therefore, the replenishment of satellite cell through cellular transplantation has been expected as a potential treatment for PMA. However, SCs are known as a heterogeneous population, composed by cells of different characters. In order to achieve the goal of satellite cell transplantation, a further characterization of satellite cell questing the optimal condition for transplant is indispensable. The Low Energy Laser Irradiation (LELI) is known to able to promote the proliferation of satellite cell, but does this effectiveness presents equally on satellite cells in fast and slow twitch muscles has not been confirmed.
[Methodology]
Here, we used primary culture cells of fast twitch muscle of Tibia (TA) and slow twitch muscle of soleus (SOL) of rat, and irradiated them with distinct irradiation conditions. These cultured cells were then co-immunostained with anti-Pax7 antibody and anti-BrdU antibody thereby to evaluate the proliferation rate of satellite cells in fast and slow twitch muscles received different irradiation conditions.
[Results]
The optimal irradiation condition for satellite cell in TA of fast muscle type and SOL of slow type to proliferate differs.
[Conclusions].
Taken together, our results suggested the existence of distinct characters between satellite cells in fast and slow twitch muscles, and this novel finding is important for realization of the satellite cell transplantation in near future.
Satellite cell (SC), known as the stem cell of skeletal muscle plays the key role in postnatal muscle regeneration. Clinically, the etiology of most progressive muscular atrophy (PMA) are known to be related to the depletion of SC. Therefore, the replenishment of satellite cell through cellular transplantation has been expected as a potential treatment for PMA. However, SCs are known as a heterogeneous population, composed by cells of different characters. In order to achieve the goal of satellite cell transplantation, a further characterization of satellite cell questing the optimal condition for transplant is indispensable. The Low Energy Laser Irradiation (LELI) is known to able to promote the proliferation of satellite cell, but does this effectiveness presents equally on satellite cells in fast and slow twitch muscles has not been confirmed.
[Methodology]
Here, we used primary culture cells of fast twitch muscle of Tibia (TA) and slow twitch muscle of soleus (SOL) of rat, and irradiated them with distinct irradiation conditions. These cultured cells were then co-immunostained with anti-Pax7 antibody and anti-BrdU antibody thereby to evaluate the proliferation rate of satellite cells in fast and slow twitch muscles received different irradiation conditions.
[Results]
The optimal irradiation condition for satellite cell in TA of fast muscle type and SOL of slow type to proliferate differs.
[Conclusions].
Taken together, our results suggested the existence of distinct characters between satellite cells in fast and slow twitch muscles, and this novel finding is important for realization of the satellite cell transplantation in near future.