[P3-56] The Intracerebroventricular Injection of MiR-146a Relieved Seizure In Rat Model With Lithium-Pilocarpine Induced Status Epilepticus
[Introduction] Status Epilepticus (SE) is a common, life-threatening neurologic emergency which confers a high degree of morbidity and mortality. Increasing evidence indicates that neuroinflammation plays a critical role in the pathogenesis of SE. MiR-146a was reported to be an important posttranscriptional inflammation-associated microRNA. The aim of this study was to investigate the effect of miR-146a in SE and the possible mechanism.
[Methodology] To study the effect of miR-146a in SE, we choose intracerebroventricular injection for rat at 3-4 weeks age, and then made the Lithium-Pilocarpine induced Status Epilepticus rat model. According to behavior observation, we made assessment about latency time, Lado grade. We performed Real Time quantitative polymerase chain reaction (qPCR) , Elisa, Western Blot on the rat hippocampus to test the express level of miR-146a, IL-1β、TNF-α, TLR4，NF-κB.
[Results] The death rate of rat with miR-146a agomir injection was observed lower than those with agomir negative control and normal saline (NS). In miR-146a antagomir injection group, the latency time before seizure occurred was shorter, the last time of seizure and the seizure degree was severe, the express level of miR-146a was obviously decreased, and both IL-1β, TNF-α, TLR4, NF-κB was significantly up-regulated, while it was in the opposite way in rat with miR-146a agomir injection.
[Conclusions] Taken together, our findings elucidate the role of inflammation in the pathogenesis of SE in the mature rat models, modulation miR-146a may be a novel therapeutic target in the treatment of SE.
[Methodology] To study the effect of miR-146a in SE, we choose intracerebroventricular injection for rat at 3-4 weeks age, and then made the Lithium-Pilocarpine induced Status Epilepticus rat model. According to behavior observation, we made assessment about latency time, Lado grade. We performed Real Time quantitative polymerase chain reaction (qPCR) , Elisa, Western Blot on the rat hippocampus to test the express level of miR-146a, IL-1β、TNF-α, TLR4，NF-κB.
[Results] The death rate of rat with miR-146a agomir injection was observed lower than those with agomir negative control and normal saline (NS). In miR-146a antagomir injection group, the latency time before seizure occurred was shorter, the last time of seizure and the seizure degree was severe, the express level of miR-146a was obviously decreased, and both IL-1β, TNF-α, TLR4, NF-κB was significantly up-regulated, while it was in the opposite way in rat with miR-146a agomir injection.
[Conclusions] Taken together, our findings elucidate the role of inflammation in the pathogenesis of SE in the mature rat models, modulation miR-146a may be a novel therapeutic target in the treatment of SE.