[P1-2-31] Effects of Phellodendron bark extract on the bacterial composition of an in vitro oral biofilm model
キーワード：歯周病、口腔細菌、バイオフィルム
Periodontal disease is associated with a dysbiotic oral microbiome. It is important to reduce the relative abundance of periodontal pathogenic bacteria in the oral microbiome to prevent the onset and progression of periodontal disease. We have previously reported that Phellodendron bark extract (PBE) can reduce the relative abundance of periodontal pathogenic bacteria to low levels in an in vitro saliva-derived multi-bacterial planktonic model. However, the effect on biofilm remains unclear. In this study, we investigated the effect of PBE on an in vitro biofilm model of subgingival plaque. Oral commensal and periodontal pathogenic bacteria such as Porphyromonas gingivalis were anaerobically cultured in a medium with or without PBE. We observed biofilm formation at the bottom of the well plate and evaluated the relative abundance of periodontal pathogenic bacteria in the biofilm. PBE treatment significantly reduced the relative abundance of periodontal pathogenic bacteria in contrast to an increase in the relative abundance of oral commensals such as Streptococci and nitrate-reducing bacteria. These effects were dose-dependent, with higher concentrations being the most effective. Our results indicate that PBE may reduce the relative abundance of periodontal pathogenic bacteria to low levels in an in vivo biofilm, thus reducing the risk of periodontal disease.