[H-CG24] Advanced life support systems with closed bio-ecosystems and thier applications

[HCG24-P03] Analysis of proteins involved in environmental stress tolerance of Shizosaccharomyces pombe spore

*Satsuki Shinoda¹, Hiroshi Katoh², Kengo Shibasaki¹, Tomoko Abe¹ (1.Tokyo Denki University , 2.Mie University)

Keywords:Shizosaccharomyces, environmental stress tolerance, spore

We are studying environmental stress tolerance in spores of Schizosaccharomyces pombe, a type of fission yeast. The spores are encased in a zoospore, and upon maturation, the zoospore breaks and the spores are released. Yeast spores are dormant cells that are known to show tolerance to various environmental stresses such as heat,1), 2) and recent environmental stress tolerance tests have shown that S. pombe spores may be able to survive in the space environment, so they have been adopted as biological material for space exposure experiments using the Exposed Facility on the International Space Station Therefore, S. pombe has been adopted as a biological material for space exposure experiments using the Exposed Facility of the International Space Station.
In this study, we aimed to elucidate the proteins that contribute to the environmental stress tolerance of S. pombe spores. We compared the expressed proteins of S. pombe spores under environmental stresses of desiccation and ultraviolet light with those of nutrient cells, and searched for proteins that are different in their expression and degradation.

References:
1) Egel, R., Arch Microbiol. 112, 109-110, 1977.
2) Kupelic, M. et al., The Molecular and Cellular Biology of the Yeast Saccharomyces Vol. 3, 889-1036, 1997.