[6p-PB5-1~12] 12.6 Nanobiotechnology

▼ [6p-PB5-7] Phase transition in a single giant DNA molecule: Differences between 1-propanol and 2-propanol aqueous solutions

〇Yue Ma¹, Yoshikawa Yuko¹, Sadakane Koichiro¹, Kenmotsu Takahiro¹, Yoshikawa Kenichi¹ (1.Doshisha Univ.)

Keywords:DNA, phase transition, high-order structure

2-Propanol is often used to isolate DNA molecules from cells through the generation of precipitation. 1-propanol is used as a solvent in the pharmaceutical industry mainly for resins and cellulose esters. In order to understand polarity of alcohol solutions’ influence on genome-sized DNA’s structure, we performed the comparative study between 1- and 2-propanols. For DNA molecules in different concentrations of 1-propanol solutions, two minima of average length appear at 60% (v/v) and 80% (v/v). In 2-propanol solutions, the average long-aixs length of DNA decreased and transited from elongated coil state to folded globule is generated around 70-75% (v/v). Above 75% (v/v), DNA maintained as folded globule and the average length of DNA molecules remains essentially the constant. The observed large difference of the effect of propanol isomers on the DNA conformation will be discussed in relation to the nano-structure of alcohol solution.