[16p-P11-1~15] 3.4 Biomedical optics

△ [16p-P11-3] Valuation of photodamage of living cells caused by short wavelength visible laser beam

〇Shohei Kawahara¹, Shintaro Namba¹, Keisuke Takahashi¹, Tetsuya Matsuyama¹, Kenji Wada¹, Ai Kawakita², Kaori Murata², Kenji Sugimoto² (1.Osaka Pre Univ. Eng, 2.Osaka Pre Univ. Bio)

Keywords:live cell imaging, laser, photodamage

For the purpose of examining the effect of blue light with respect to living cells, we carried out quantitative evaluation of phototoxic property of short-wavelength light using live cell imaging. Compared with the 405 nm laser irradiation, 450 nm laser irradiation was observed to cause a relatively lower rate of cell death. Cell death rate was confirmed to lead to 50% in the case of a 450 nm laser of 168 kJ/cm² light energy and in the case of a 405 nm laser of 72 kJ/cm² light energy, respectively.