[20p-PA1-1~7] 3.4 Biomedical optics

[20p-PA1-4] Cell cycle dependence of phototoxicity by blue laser light

〇Naohiro Kanamaru¹, Mikiya Yamaguchi¹, Tetsuya Matsuyama¹, Kenji Wada¹, Koichi Okamoto¹, Ai Kawakita², Kaori Murata², Kenji Sugimoto² (1.Engineering Osaka Pref. Univ., 2.Life and Environmental Sciences Osaka Pref. Univ.)

Keywords:blue light, cell cycle, live cell imaging

Extensive exposure to short-wavelength light is known to cause diseases such as age-related macular degeneration (AMD) and retinal damage. We use LEDs and semiconductor lasers for live cell imaging as a light source, and conduct quantitative evaluation of the phototoxicity of blue light on live cells. In this study, cells were irradiated with laser light by changing the irradiation wavelength, irradiation energy, and cell cycle, and the phototoxicity was compared based on the cell survival rate.