[10p-PA3-1~21] 12.7 Biomedical Engineering and Biochips

[10p-PA3-19] Development of microfluidic cell sorting system with Weir Structure-Based Filter

〇Tsuyoshi Ono¹, Wilfred Espulgar¹, Masato Saito^3,1, Syohei Koyama², Hyota Takamatsu², Atsushi Kumanogou², EiiChi Tamiya¹ (1.Osaka Univ Engineering, 2.Osaka Univ Medicine, 3.AIST PhotoBIO-OIL)

Keywords:microfluidic device

This report presents the cell sorting capability of the weir structured-based filters in the designed microfluidic chip. Sorting and separation of cells from a heterogeneous mixture is a vital process in any cell-related research. The biophysical characteristics of the cell such as morphology, size, density, and dielectric susceptibility are commonly utilized in a label-free sorting method but often lacks specificity. The use of labels or biological markers is common alternative but succeeding processes can have an influence on the cellular phenotype. In this study, the cell sorting capability is associated to the deterministic cell rolling of related to the rapid formation and rapid dissociation of adhesive bond on a contact surface. The weir structure is subjected to different cells available to test its compatibility and with the ultimate goal of sorting the type of cells in a blood, specifically, white blood cells. The design has been further modified based on the results. With this result, sorting method can be realized that can be utilized for low concentration cell volume; a common downside of commercialized machines and protocols.