[10p-S421-1~9] 12.7 Biomedical Engineering and Biochips

[10p-S421-7] Highly sensitive detection of viral nucleoprotein using VHH antibody and surface plasmon resonance

〇Hiroto Yanagawa¹, Kazuaki Nishio¹, Noriko Shimba¹, Emina Ikeuchi¹, Tatsuro Kawamura¹, Masahiko Shioi¹, Kohei Tsumoto² (1.Panasonic Corp., 2.The Univ. of Tokyo)

Keywords:biosensor, plasmon, antibody

We developed a highly sensitive detection technology for nucleoprotein of influenza virus, which is applicable to the detection of a trace amount of influenza virus in the air. In order to enhance sensitivity, we examined the detection method using an antigen-antibody reaction by a new small antibody (VHH antibody), and surface plasmon resonance to enhance a fluorescent dye immobilized to VHH antibody. As a result, it was demonstrated that the nucleoprotein can be detected with a concentration of 1 pM by using VHH antibody, plasmonic substrate fabricated by sputtering deposition and mixed self-assembled monolayer having ethylene glycol.