[11p-M121-1~10] Interplay and integration of experiments and calculations in nanobiotechnology: lipid membrane and membrane proteins

[11p-M121-8] Lateral diffusion of lipids and phase separation in freestanding membrane suspended over microwells

〇Koji Sumitoro¹, Azusa Oshima², Hiroshi Nakashima², Norio Inui¹ (1.Univ. of Hyogo, 2.NTT BRL)

Keywords:lateral diffusion, phase separation, lipid bilayer

In this paper, we discuss the lateral diffusion of lipids and phase separation between liquid-ordered (Lo) and liquid-crystalline (La) phase separation at the freestanding lipid bilayer suspended over microwells. We found that the lateral diffusion through the boundary between the freestanding part and the supported part on the substrate is anisotropic. It is also indicated the existence of the large diffusion barrier at the boundary. These play an important role in the formation and retention of phase separation at the freestanding membrane.