[9a-W242-1~12] 12.6 Nanobiotechnology

[9a-W242-3] Single-virus identification using biorecognition nanopore

〇Makusu Tsutsui¹, Akihide Arima¹, Ilva Harlisa², Takeshi Yoshida¹, Masayoshi Tanaka², Kazumichi Yokota¹, Wataru Tonomura¹, Takashi Washio¹, Mina Okochi², Tomoji Kawai¹ (1.ISIR, Osaka Univ., 2.Tokyo Inst. Tech.)

Keywords:nanopore, Artificial intelligence, nanofluidics

We report on development of biorecognition solid-state nanopores for selective detections of viruses. We fabricated Au nanopores on an ultrathin Si3N4 membrane using MEMS technology. On the Au surface, we chemically adhered peptide molecules having relatively high affinity to haemagulutinin on influenza virions. We found that the resistive pulse width tend to be longer as we used peptide probes having higher affinity to the viral surface proteins, which facilitated influenza discrimination under machine learning pattern analyses.