[13a-D221-1~12] 6.4 Thin films and New materials

▲ [13a-D221-7] SPR sensor system for lectin-recognition using molecular imprinting hydrogel
with carbohydrate ligands

〇(P)Rinyarat Naraprawatphong¹, Nagahiro Saito^1,2,3, Akifumi Kawamura⁴, Takashi Miyata⁴ (1.Nagoya Univ., 2.JST-SICORP, 3.JST-OPERA, 4.Kansai Univ.)

Keywords:Surface Plasmon Resonance, molecular imprinting

Surface Plasmon Resonance is one kind of optical biosensors which can detect changes in the reflective index according to the biomolecular interaction between a target molecule and ligands molecule that attached on the chip surface. Molecularly imprinted hydrogel layers with lectin-recognition sites were formed on the SPR sensor chips via surface initiated atom transfer radical polymerization comnined with molecular imprinting in order to develop SPR sensor systems for sensitively and selectively detecting a target protein. The lectin imprinted hydrogel showed the larger SPR signal change in response to a target protein than nonimprinted hydrogel. It was attributed to the strong affinity constant of the lectin imprinted hydrogel layer for the target lectin. These results suggest that molecular recognition sites for the lectin were formed within the hydrogel layers by molecular imprinting. On te other hand, the SPR signal change of the lectin impronted hydrogel in the presence of another lectin was very small. The fascinating properties of such molecularly impronted hydrogel layer sensor chips can provide the useful tools to produce biosensors with a wide variety of uses.