[20a-P06-1~11] 12.7 Biomedical Engineering and Biochips

[20a-P06-8] Virucidal effect of monogalactosyl diacylglyceride (MGDG) against SARS-CoV-2 by advanced plaque assay

〇Toshio Kawahara¹, Kyoko Hayashi¹, Satomi Asai², Kazuo Umezawa², Hidefumi Kakizoe², Hayato Miyachi², Masanobu Morita³, Takaaki Akaike³, Hitoshi Kuno⁴, Satoko Komatsu⁴, Takumi Watanabe¹ (1.Chubu Univ., 2.Tokai Univ., 3.Tohoku Univ., 4.DENSO)

Keywords:detection time, infectious virus, natural product

Background: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the causative agent of coronavirus disease 2019 (COVID-19) and is capable of human-tohuman transmission and rapid global spread. Thus, the establishment of high-quality viral detection and quantification methods, and the development of anti-SARS-CoV-2 agents are critical.
Methods: Here, we present the rapid detection of infectious SARS-CoV-2 particles using a plaque assay with 0.5% agarose-ME (Medium Electroosmosis) as an overlay medium.
Results: The plaques were capable of detecting the virus within 36–40 h postinfection. In addition, we showed that a monogalactosyl diacylglyceride isolated from a microalga (Coccomyxa sp. KJ) could inactivate the clinical isolates of SARS-CoV-2 in a time- and concentration-dependent manner.
Conclusions: These results would allow rapid quantification of the infectious virus titers and help develop more potent virucidal agents against SARS-CoV-2.