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[II-18-09] Glycero- and sphingo-phospholipid differ in their effects on the function of the tight junction in human epidermal keratinocyte
[Introduction] Milk sphingomyelin (SM) and egg phosphatidylcholine (PC) are typical functional phospholipid (PL) derived from livestock products. The previous study has shown that adding some kinds of functional PL increases tight junction (TJ) protein expression and transepithelial electrical resistance (TER) in NHEK cells. This study investigated the effect of SM and PC derived from livestock products to TJ in NHEK. [Methods] After seeding NHEK into Transwell plate, milk SM (SM), egg PC (EPC), or soybean PC (SPC) (0.2 to 1.25 mM) were administered to the apical wells, and the cells were incubated for 4 days. TER was measured every day. After washing with PBS, cells were collected and measured levels of TJ proteins by Immunoblot method. [Results] TER and protein expressions of Claudin-1 and ZO-1 significantly decreased in the addition of SPC and EPC compared with the control without PL, but no significant difference in SM. [Conclusion] SM has sphingosine backbone, while SPC and EPC have glycerol backbone, although both of them contain choline. These results show that the difference in the molecule structure of PL may affect the TJ protein expression in skin.