[I-15-20] Bacterial and fungal communities of corn silage from short- and long-term bunker silos
To elucidate how bacterial and fungal changes involve in fermentation process within bunker silos. Silage samples were collected from five dairy farms. PCoA (Principal Coordinate Analysis) analysis based on the fermentation products indicated that one group showed low pH value, high concentrations of lactic acid and ethanol, low concentration of acetic acid; another group showed high pH value, high concentrations of acetic acid and 1-propanol, and low concentrations of lactic acid and ethanol. PCoA analysis based on the bacterial or fungal genus indicated that the bacterial community was more variable than fungal community in bunker silos. For bacteria, most samples were clustered together by the predominant genera of Lactobacillus. 4 samples were clustered together by dominant genus of Lactobacillus andAeriscardovia. 4 samples were individually separated by Acetobacter. For fungi, most samples were clustered one group by dominant genus of Candida. 4 samples were separated by Monascus or Kazachstania. Characterization of microbiota by NGS is helpful for detecting unusual bacteria and fungi in corn silage fermentation.