畜産物利用2

[V-16-21] Immunomodulatory potential of EPS produced by LAB for the dairy industry

*HAFIZA MAHREEN MEHWISH¹, RIAZ RAJOKA MUHAMMAD SHAHID¹, Kober AKM Humayun¹, Tomokiyo Mikado, Zhou Binghui, Wakako Ikeda-Ohtsubo^1,2, Kitazawa Haruki^1,2 (1. Laboratory of Animal Food Function, Tohoku University , 2. Tohoku University)

Objective : Extracellular polysaccharides produced by lactic acid bacteria exert their immunological ability. Therefore, in this study, to elucidate the detailed mechanisms for immunological functions of EPS-producing LAB. Methods : An EPS, designated as MM89-EPS, was isolated from Lactiplantibacillus plantarum from milk and investigated their immunomodulatory activity. Results : MM89-EPS displayed excellent water solubility and capacities to retain water and oil due to its porous structure. MM89-EPS exhibited no significant cytotoxicity on RAW264.7 cells and showed strong immunomodulatory activity by increasing phagocytosis, acid phosphatase activity, and cytokine production in RAW264.7 cells. Furthermore, an in vivo study revealed that splenic indices, intestinal IgA levels, serum cytokine levels, and lymphocyte proliferation were increased in an MM89-EPS-treated cyclophosphamide-induced immunosuppressed mouse model. 1) H. M. Mehwish., M.S.R. Rajoka et al., Food Chem. (2022).