[P-067] ラット実験的歯周炎におけるテアフラビンの組織炎症および骨吸収抑制効果に関する研究
The effects of theaflavins on tissue inflammation and bone resorption on experimental periodontitis in rats
研修コード：2504
Keywords:テアフラビン、実験的歯周炎、歯槽骨吸収、炎症
Background: Theaflavins, as a kind of polyphenol derived from black tea, has potent inhibitory effects on inflammation and bone resorption. The aim of this study was to evaluate the effect of theaflavins on periodontal destruction induced by the experimental periodontitis in rats.
Material and methods: Eighteen rats were divided into 3 groups: Control（vehicle; without ligation）, Ligature（vehicle; with ligation）, and TF（theaflavins: 100 mg/ml; with ligation）. To induce experimental periodontitis, the sterile 3-0 silk ligatures were placed around maxillary first molars（M1）bilaterally. After ligature placement, rats were topically applied with 100 μl of vehicle or theaflavins around M1 on everyday. Rats were euthanized at 1 week after ligature placement.
The left side of maxilla was measured for bone resorption by micro-CT, and the gingival tissue was removed for investigating mRNA expression by RT-PCR. The right side was histologically analyzed by H-E and tartrate-resistant acid phosphatase staining.
Results: Alveolar bone loss in TF group was significantly less than in Ligature group. Ligature group also showed greater numbers of inflammatory cells in the gingival tissues and osteoclasts on alveolar bone when compared with Control group, but these numbers decreased in TF group. Furthermore, the gene expression levels of IL-6, RANKL, and MMP-9, enhanced in ligature group, were markedly suppressed in TF group.
Conclusion: The present study suggested that topical application of theaflavins had potential effect against bone loss and inflammatory response on the experimental periodontitis in rats.
Material and methods: Eighteen rats were divided into 3 groups: Control（vehicle; without ligation）, Ligature（vehicle; with ligation）, and TF（theaflavins: 100 mg/ml; with ligation）. To induce experimental periodontitis, the sterile 3-0 silk ligatures were placed around maxillary first molars（M1）bilaterally. After ligature placement, rats were topically applied with 100 μl of vehicle or theaflavins around M1 on everyday. Rats were euthanized at 1 week after ligature placement.
The left side of maxilla was measured for bone resorption by micro-CT, and the gingival tissue was removed for investigating mRNA expression by RT-PCR. The right side was histologically analyzed by H-E and tartrate-resistant acid phosphatase staining.
Results: Alveolar bone loss in TF group was significantly less than in Ligature group. Ligature group also showed greater numbers of inflammatory cells in the gingival tissues and osteoclasts on alveolar bone when compared with Control group, but these numbers decreased in TF group. Furthermore, the gene expression levels of IL-6, RANKL, and MMP-9, enhanced in ligature group, were markedly suppressed in TF group.
Conclusion: The present study suggested that topical application of theaflavins had potential effect against bone loss and inflammatory response on the experimental periodontitis in rats.