[2SP-26-15] Application of chromosome engineering technology (5): Construction of top-down HAC derived from chromosome 21 in human iPS cells by megabase deletion using genome editing (CRISPR/Cas9)

^○Masaya Egawa¹, Narumi Uno¹, Hitomaru Miyamoto², Yusuke Ohkame³, Rina Komazaki¹, Yuki Ishizu¹, Yusaku Okano¹, Taku Yunomae¹, Yasuhiro Kazuki^2,3,4, Kazuma Tomizuka¹ (1.Lab. of Bioeng., Sch. of Life Sci., Tokyo Univ. of Pharm and Life Sci, 2.Div. of Chromo. Biomed Eng., Grad. Sch. of Med. Sci., Tottori Univ., 3.CERC, Tottori Univ., 4.Chr. Eng. Group, ExCELLS)

The 45th Annual Meeting of the Molecular Biology Society of Japan

[1SP-24-05] Application of chromosome engineering technology (6): Construction of human iPS cells with partial monosomy of chromosome 21 and search for essential regions

^○Rina Komazaki¹, Yuki Ishizu¹, Masaya Egawa¹, Yusaku Okano¹, Taku Yunomae¹, Yusuke Ohkame², Shuta Takata², Chihiro Yoshimatsu³, Kyoutarou Yamazaki³, Teruhiko Suzuki⁴, Yasunori Aizawa⁵, Narumi Uno¹, Yasuhiro Kazuki^2,3,6, Kazuma Tomizuka¹ (1.Lab. of Bioeng., Sch. of Life Sci., Tokyo Univ. of Pharm and Life Sci., 2.CERC, Tottori Univ., 3.Div. of Chromo. Biomed. Eng., Grad. Sch. of Med. Sci., Tottori Univ., 4.Stem Cell Proj., Tokyo Metropol. Inst. Med. Sci., 5.Sch. Life Sci. Tech., Tokyo Tech., 6.Chr. Eng. Group, ExCELLS)

The 45th Annual Meeting of the Molecular Biology Society of Japan

[1SP-20-04] Application of chromosome engineering technology (7): Generation of elephantized mice with elephant-specific gene functions and analysis of their contribution to carcinogenesis inhibition

^○Kyotaro Yamazaki¹, Yasuhiro Kazuki^1,2,3 (1.Div. of Chr. Biomed. Eng., Grad. Sch. of Med. Sci., Tottori Univ., 2.CERC, Tottori Univ., 3.Chr. Eng. Group, ExCELLS)

The 45th Annual Meeting of the Molecular Biology Society of Japan

[1SP-21-09] Application of chromosome engineering technology (9): Generation and analysis of humanized mice retaining the mouse artificial chromosome harboring HLA class I cluster

^○Nanami Kishima¹, Takashi Moriwaki^1,2, Kanako Kazuki², Wakana Nakagawa¹, Narumi Uno³, Kazuma Tomizuka³, Teruhiko Suzuki⁴, Yasuhiro Kazuki^1,2,5 (1.Div. of Chr. Biomed. Eng., Grad. Sch. of Med. Sci., Tottori Univ., 2.CERC, Tottori Univ., 3.Lab. of Bioeng., Sch. of Life Sci., Tokyo Univ. of Pharm. and Life Sci., 4.Stem Cell Proj., Tokyo Metropol. Inst. Med. Sci., 5.Chr. Eng. Group, ExCELLS)

The 45th Annual Meeting of the Molecular Biology Society of Japan

[1SP-22-09] Application of chromosome engineering technology (10):Development of human IgH/Igλantibody-producing mice by using artificial chromosome.

^○Kazuto Shimoya¹, Takashi Moriwaki^2,1, Satoshi Abe³, Akane Okada¹, Kanako Kazuki², Yasuhiro Kazuki^4,2,1 (1.Div. of Chr. Biomed. Eng., Grad. Sch. of Med. Sci., Tottori Univ., 2.CERC, Tottori Univ., 3.Trans Chromosomics Inc., 4.Chr. Eng. Group, ExCELLS)

The 45th Annual Meeting of the Molecular Biology Society of Japan

[1SP-21-05] Application of chromosome engineering technology (12): Analysis of antibody affinity maturation in fully human antibody producing mice

^○Takashi Moriwaki^1,2, Hiroyuki Satofuka², Satoshi Abe³, Hiroshi Tanaka³, Kayoko Morimoto³, Kazuma Tomizuka⁴, Mitsuo Oshimura³, Yoshihiro Baba⁵, Yasuhiro Kazuki^1,2,6 (1.Div. of Chr. Biomed. Eng., Grad. Sch. of Med. Sci., Tottori Univ., 2.CERC, Tottori Univ., 3.Trans Chromosomics Inc., 4.Lab. of Bioeng., Sch. of Life Sci., Tokyo Univ. of Pharm. and Life Sci., 5.Div. Immunol. and Genome Biol., Depart. of Mol. Genet., Med. Inst. of Bioreg., Kyushu Univ., 6.Chr. Eng. Group, ExCELLS)

The 45th Annual Meeting of the Molecular Biology Society of Japan

[1SP-21-17] High-order structure of DNA-protein complex by chemically modified histone-like protein

^○Natsumi Araya, Fumiaki Tomoike, Tetsuji Okada (Dept. of Biol. Sci., Fac. of Sci., Gakusyuin Univ.)

The 45th Annual Meeting of the Molecular Biology Society of Japan

[1SP-21-18] A pathway to produce piRNAs from endogenous protein-coding sequences supports Drosophila spermatogenesis

^○Taichiro Iki, Shinichi Kawaguchi, Toshie Kai (Grad. Sch. of FBS (Frontier Biosciences), Osaka Univ)

The 45th Annual Meeting of the Molecular Biology Society of Japan

[1SP-22-01] Inhibiting Cross Talk Between HCV NS5A and NFkβ1/Bcl-2/HIF-1a Pathways with Medicinal Plant Extracts

^○Hafiza Ishrat Fatima¹, Bushra Ijaz², Somayya Tariq², Landry Brice Koloko² (1.Center for iPS Cell Research and Application, Kyoto University, 2.Centre for Excellence in Molecular Biology, University of the Punjab)

The 45th Annual Meeting of the Molecular Biology Society of Japan

[1SP-22-02] Genome-wide mining of non-coding RNAs in the food-borne pathogen Vibrio parahaemolyticus using direct RNA sequencing

^○Mohamad Al kadi¹, Shigeaki Matsuda², Eiji Ishii², Hiroyuki Terashima³, Tetsuya Iida², Daisuke Okuzaki^1,4,5, Toshio Kodama³ (1.Single cell genomics, Human Immunology, IFReC, Univ. of Osaka, 2.Dept. of Bact. Infections, RIMD, Univ. of Osaka, 3.Dept. of Bact., Inst. of Tropical Med., Univ. of Nagasaki, 4.Genome Info. Res. Center, RIMD, Univ. of Osaka, 5.CiDER, Univ. of Osaka)

The 45th Annual Meeting of the Molecular Biology Society of Japan