[2P-0377] Development of T cell engineering technology based on artificial mRNA vector

○Ryo Miyama¹, Hiroki Tanaka¹, Yuta Nakai², Yu Sakurai¹, Shinya Tamagawa², Kota Tange², Hidetaka Akita¹ (1.Graduate School of Pharmaceutical Science, Chiba University, 2.DDS Research Laboratory, NOF CORPORATION)

The 43rd Annual Meeting of the Molecular Biology Society of Japan

[2P-0378] Evaluation of combinatory effects of Plasmodium circumsporozoite protein and complement regulatory protein expression of recombinant baculovirus vectors

Chiaki Kawabata¹, Yusuke Kawai¹, ○Takahiko Tamura¹ (1.Sch. of Pharmacy, Kanazawa Univ. )

The 43rd Annual Meeting of the Molecular Biology Society of Japan

[2P-0379] Comprehensive analysis of sequence features of CRISPR-Cas9 off-target sites using guide RNAs with low specificity

○Takuma Yamashita¹, Yuki Naito², Takenori Yamamoto¹, Tokuyuki Yoshida¹, Takao Inoue¹, Eriko Uchida¹ (1.NIHS, 2.DBCLS)

The 43rd Annual Meeting of the Molecular Biology Society of Japan

[2P-0380] Synergistic upregulation of target genes by TET1 and VP64 in the dCas9-SunTag platform.

○Sumiyo Morita¹, Takuro Horii¹, Mika Kimura¹, Izuho Hatada¹ (1.Biosignal Genome Resource Center, IMCR, Gunma University)

The 43rd Annual Meeting of the Molecular Biology Society of Japan

[2P-0381] Immunodeficient Mouse Model of Fabry Disease for Development of New Cell Therapy

○Ittetsu Nakajima^1,2, Yuichiro Miyaoka¹ (1.Regenerative Medicine Project, Tokyo Metropolitan Institute of Medical Science, 2.Dept. of Med. Den. Sci., Grad. Sch. of Med. Den. Sci., Univ of Tokyo Medical and Dental)

The 43rd Annual Meeting of the Molecular Biology Society of Japan

[2P-0382] Optimization of CRISPR genome editing in mouse liver using hydrodynamic delivery methods

○Jurai Imafuku¹, Aki Kurosaki¹, Chisa Okada², Youko Kameyama², Kenya Kamimura³, Dexi Liu⁴, Hiromi Miura¹, Masato Ohtsuka¹ (1.Dept. of Mol. Life Sci., Div. of Basic Med. Sci. & Mol. Med.,Univ. of Tokai, 2.Dept. of Cell Biol. and Hist., Sup. Cent. for Med. Res. & Edu.,Dept. of Res. Prom.,Univ. of Tokai, 3.Div. of Gastroenterol. & Hepatol., Grad. Sch. of Med. & Dental Sci.,Univ. of Niigata, 4.Dept. of Pharm. & Biomed. Sci., College of Pharm., Univ. of Georgia)

The 43rd Annual Meeting of the Molecular Biology Society of Japan

[2P-0383] Sequence features of efficient CRISPR-Cas9 crRNAs for zebrafish genome editing using crRNA-tracrRNA-Cas9 ribonucleoprotein complex

○Keita Okada¹, Takuya Takada¹, Kanae Aoki¹, Teruyuki Tabei¹, Katunori Imai¹, Yuki Bonkohara¹, Yusuke Kamachi¹ (1.Sch. of Env. Sci. and Eng., Kochi Univ. of Tech.)

The 43rd Annual Meeting of the Molecular Biology Society of Japan

[2P-0384] Evaluation of off-target effects of gapmer antisense oligonucleotides using human cells

○Tokuyuki Yoshida¹, Kiyomi Sasaki¹, Satoshi Obika², Takao Inoue¹ (1.NIHS, 2.Grad. Sch. Pharm. Sci., Osaka Univ)

The 43rd Annual Meeting of the Molecular Biology Society of Japan

[2P-0385] Development of a SARS-CoV-2 genome subtyping method by using ORNi-PCR

○Norishige Yamada¹, Toshitsugu Fujita^1,2, Hodaka Fujii^1,2 (1.Epigeneron, Inc., 2.Dept. Biochem. Genome Biology., Grad. Sch. Med., Hirosaki Univ.)

The 43rd Annual Meeting of the Molecular Biology Society of Japan

[2P-0386] Improved Target-AID base editing with minimized off-target and molecular size

○Ang Li¹, Hitoshi Mitsunobu¹, Akihiko Kondo^1,2, Keiji Nishida¹ (1.Kobe University, 2.RIKEN)

The 43rd Annual Meeting of the Molecular Biology Society of Japan