[2P-0397] Genome editing with the donor plasmid equipped with synthetic crRNA-target sequence

○Riki Ishibashi^1,2, Kota Abe^1,2, Nanami Ido^1,2, Satsuki Kitano¹, Hitoshi Miyachi¹, Fumiko Toyoshima^1,2 (1.Lab. of Tissue Homeo., Inst. for Ftont. Life and Med. Sci., Kyoto Univ., 2.Lab. of Cell Divi. and Dif., Dept. of Mam. Regu. Net., Grad. Sch. of Biostudies, Kyoto Univ.)

The 43rd Annual Meeting of the Molecular Biology Society of Japan

[2P-0398] MaChIAto: detailed profiling tool for various genomic features affecting the efficacy of CRISPR-based genome editing

○Kazuki Nakamae¹, Mitsumasa Takenaga², Shota Nakade^3,4, Ichiro Nazuka⁵, Akinori Awazu², Naoaki Sakamoto², Tetsushi Sakuma², Takashi Yamamoto^1,2 (1.Genome Editing Innovation Center. Hiroshima Univ., 2.Grad. Sch. Integr. Sci. for Life, Hiroshima Univ. , 3.Grad. Sch. Sci., Hiroshima Univ., 4.Present address: Massachusetts Inst. Tech. (MIT), 5.TOPPAN PRINTING CO., LTD., Information and Communication Div.)

The 43rd Annual Meeting of the Molecular Biology Society of Japan

[2P-0399] Development of translational activation tool using designer PPR (Pentatricopeptide Repeat) proteins

○Nei Hei¹, Sayuri Kuge¹, Taizo Tamura¹, Takahiro Nakamura¹ (1.Dept. of Biosci. & Biotec., Sch. of Agricul., Kyushu Univ.)

The 43rd Annual Meeting of the Molecular Biology Society of Japan

[2P-0400] Transgene-free genome editing using removable Platinum TALEN plasmids with a yeast centromere and autonomous replication sequence (ARS) in oleaginous microalga, Nannochloropsis

○Tomokazu Kurita¹, Keishi Moroi², Masako Iwai³, Kumiko Okazaki¹, Seiji Nomura⁴, Fumihiko Saito⁴, Akihide Takami⁴, Atsushi Sakamoto¹, Hiroyuki Ohta³, Tetsushi Sakuma¹, Takashi Yamamoto¹ (1.Grad. Sch. of Inte. Sci. for Life, Hiroshima Univ., 2.Grad. Sch. of Sci.,Hiroshima Univ., 3.Sch. of Life Sci. and Tech., Tokyo Inst. of Tech., 4.Mazda Motor Corporation)

The 43rd Annual Meeting of the Molecular Biology Society of Japan

[2P-0401] Optimization of transcriptional activation platform based on Class 1 type I-E CRISPR-Cas system

○Shiho Nakamura¹, Atsushi Kunii², Kazuto Yoshimi³, Tomoji Mashimo³, Takashi Yamamoto¹, Tetsushi Sakuma¹ (1.Dept. Integr. Sci. for Life, Grad. Sch. Integr. Sci. for Life, Hiroshima Univ., 2.Dept. Math. Life Sci., Grad. Sch. Sci., Hiroshima Univ., 3.Inst. Med. Sci., Univ. of Tokyo )

The 43rd Annual Meeting of the Molecular Biology Society of Japan

[2P-0402] Zn²⁺-dependent DNAzymes that cleave all combinations of ribonucleotides

○Rika Inomata^1,2, Makoto Miyagishi^1,2, Jing Zhao^2,3 (1.Life Sci. Inovation, Univ. of Tsukuba, 2.Mol. Comp. Phys., Health and Med., AIST, 3.Dept. of Plant Path., Nanjing Agri. Univ)

The 43rd Annual Meeting of the Molecular Biology Society of Japan

[2P-0403] Splitting-off of 1 Mb Sub-Chromosome in Escherichia coli and its in vitro Amplification

○Tatsuya Yoneji¹, Takahito Mukai¹, Masayuki Su'etsugu¹ (1.Dept. of Life Sci., Grad. Sch. of Sci., Rikkyo Univ.)

The 43rd Annual Meeting of the Molecular Biology Society of Japan

[2P-0404] The outline of NBRP-immunodeficient rats and Support Animal Model Platform

○Kosuke Hattori¹, Yuko Yamauchi¹, Miho Hoshi¹, Jinxi Wang¹, Kazuto Yoshimi^1,2, Tomoji Mashimo^1,2 (1.Division of Animal Genetics, Laboratory Animal Research Center, The Institute of Medical Science, The University of Tokyo, 2.Division of Genome Engineering, Center for Experimental Medicine and Systems Biology, The Institute of Medical Science, The University of Tokyo)

The 43rd Annual Meeting of the Molecular Biology Society of Japan

[2P-0405] Non-enzymatic accurate and precise regulation of gene expression using ultrafast RNA photocrosslinking

○Ayumu Hirano¹, Junichi Mihara¹, Yung-Chun Hung¹, Shigetaka Nakamura¹, Kenzo Fujimoto¹ (1.Dept. of Adv. Sci. and Tech. Sch. of Adv. Sci. and Tech., JAIST)

The 43rd Annual Meeting of the Molecular Biology Society of Japan

[2P-0406] High-sensitivity detection of Escherichia coli 16s rRNA 627-644 region using ultrafast RNA photocrosslinking

○Yasuha Watanabe¹, Nanami Watanabe¹, Kenzo Fujimoto¹ (1.Dept of Adv. Sci. and Tech. Sch. of Adv. Sci. and Tech., JAIST)

The 43rd Annual Meeting of the Molecular Biology Society of Japan