[P2-86] Neuroprotective effect of Vitamin E and vitamin C by inhibiting autophagy of the immature epileptic rat hippocampus
[Introduction] In the present study, we evaluated the neuroprotective effect of autophagy inhibitors Vitamin E and vitamin C by inhibiting autophagy of the immature epileptic rat hippocampus.
[Methodology] After successful establishment of status epilepticus model of immature rats, rats were randomly divided into several groups: Control group (Ctrl), status epilepticus group (SE), vitamin E group (VE) and vitamin C group (VC) and vitamin E + vitamin C group (EC). Western blotting and immunofluorescence staining were applied to investigate the autophagy associated protein expression 24 hours after seizure onset, including ratio of LC3 II to LC3 I and beclin-1. Haematoxylin & Eosin staining and Nissl staining were performed to evaluate pathology features of hippocampus neurons of each group 30 days after treatment.
[Results] The results of western blotting and immunofluorescence staining suggested ratio of LC3 II to LC3 I and beclin-1 in SE were both obviously increased 24 hours after seizure onset. However, the autophagy associated protein expression in VE, VC and EC were lower relatively. Neurons of Ctrl were clear and complete with normal nucleolus, well-distributed karyotin and rich nissl bodies in kytoplasm. Neurons in SE were swelling and in irregular arrangement. Most pyramid cells were normal and only a few neurons showed condensation of chromatin and fuzzy outline in VE, VC and EC. The surviving neurons in VE, VC and EC were more than in SE (p<0.05).
[Conclusions] Autophagy inhibitors such as vitamin E and vitamin C could alleviate the injuries induced by seizures through inhibiting autophagy with effect.
[Methodology] After successful establishment of status epilepticus model of immature rats, rats were randomly divided into several groups: Control group (Ctrl), status epilepticus group (SE), vitamin E group (VE) and vitamin C group (VC) and vitamin E + vitamin C group (EC). Western blotting and immunofluorescence staining were applied to investigate the autophagy associated protein expression 24 hours after seizure onset, including ratio of LC3 II to LC3 I and beclin-1. Haematoxylin & Eosin staining and Nissl staining were performed to evaluate pathology features of hippocampus neurons of each group 30 days after treatment.
[Results] The results of western blotting and immunofluorescence staining suggested ratio of LC3 II to LC3 I and beclin-1 in SE were both obviously increased 24 hours after seizure onset. However, the autophagy associated protein expression in VE, VC and EC were lower relatively. Neurons of Ctrl were clear and complete with normal nucleolus, well-distributed karyotin and rich nissl bodies in kytoplasm. Neurons in SE were swelling and in irregular arrangement. Most pyramid cells were normal and only a few neurons showed condensation of chromatin and fuzzy outline in VE, VC and EC. The surviving neurons in VE, VC and EC were more than in SE (p<0.05).
[Conclusions] Autophagy inhibitors such as vitamin E and vitamin C could alleviate the injuries induced by seizures through inhibiting autophagy with effect.