[P2-87] miR-105 inhibits epilepgogenesis by suppressing Atg-7 in immature rat
[Introduction] In the present study, we evaluated the autophagy level and miRNAs expression in hippocampus of immature rat following status epilepticus (SE), and tried to provide clues for further examination of the regulation of epilepsy pathogenesis through miRNA-autophagy pathway.
[Methodology] The miRNA expression profile in hippocampus of immature rat following SE was detected by miRNA array. QRT-PCR was employed to prove the accuracy of miRNA array and evaluate dynamics expression of deregulated miRNAs. Moreover, targets of these deregulated miRNAs were analyzed using miRWalk database. Western blotting and immunofluorescence staining were applied to investigate the autophagy associated protein expression.
[Results] Through miRNA array and differential analysis, we have selected 30 up-regulated miRNAs and 19 down-regulated miRNAs in rat hippocampus following SE. QRT-PCR detection of six randomly selected upregulated miRNAs (miR-31b, 145-3p and let-7d-3p) and down regulated miRNAs (miR-96-5p, 105, 301b-3p) proved the accuracy of the miRNA array. We then focused our analysis on miR-105, a novel miRNA which was not reported to be involved in epilepsy previously. Target predication with miRWalk database demonstrated that Atg-7, a member of autophagy related protein family, is the direct target of miR-105. Importantly, there was an inverse correction between miR-105 and Atg-7 expression in different time points post SE (2, 6, 12, 24, 48 and 72h post SE).
[Conclusions] Our results suggest that miR-105 might regulate autophagy in the brain of SE rats through directly targeting Atg-7, provided autophagamiRNAs as potential targets for SE therapy.
[Methodology] The miRNA expression profile in hippocampus of immature rat following SE was detected by miRNA array. QRT-PCR was employed to prove the accuracy of miRNA array and evaluate dynamics expression of deregulated miRNAs. Moreover, targets of these deregulated miRNAs were analyzed using miRWalk database. Western blotting and immunofluorescence staining were applied to investigate the autophagy associated protein expression.
[Results] Through miRNA array and differential analysis, we have selected 30 up-regulated miRNAs and 19 down-regulated miRNAs in rat hippocampus following SE. QRT-PCR detection of six randomly selected upregulated miRNAs (miR-31b, 145-3p and let-7d-3p) and down regulated miRNAs (miR-96-5p, 105, 301b-3p) proved the accuracy of the miRNA array. We then focused our analysis on miR-105, a novel miRNA which was not reported to be involved in epilepsy previously. Target predication with miRWalk database demonstrated that Atg-7, a member of autophagy related protein family, is the direct target of miR-105. Importantly, there was an inverse correction between miR-105 and Atg-7 expression in different time points post SE (2, 6, 12, 24, 48 and 72h post SE).
[Conclusions] Our results suggest that miR-105 might regulate autophagy in the brain of SE rats through directly targeting Atg-7, provided autophagamiRNAs as potential targets for SE therapy.