[P2-88] The Influence of The Epilepsy Drug PTX on Zebrafish Larvae Behaviors: A Comparison Study of PTZ
[Introduction] Larval zebrafish (Danio rerio) have been suggested as a high-throughput experimental animal model for epilepsy-related genetic and developmental studies. We used 5dpf zebrafish larvae to study the behavioral manifestations induced by the seizure-inducing drugs, picrotoxin (PTX) and pentylenetetrazole (PTZ).
[Methodology] The behavioral manifestations to the seizure-inducing drugs PTX (1 μM, 5 μM, 25 μM, 125 μM, or 625 μM) or PTZ (1 mM, 2 mM, 4 mM, 8 mM, or 16 mM) under light-dark conditions were studied using zebrafish larvae at 5 days post-fertilization (dpf). Two behavioral parameters, locomotor activity and thigmotaxis behavior, were analyzed.
[Conclusions] We conclude that PTX treatment increased locomotion and thigmotaxis in 5 dpf zebrafish larvae under continuous illumination and the locomotion of PTX-treated zebrafish was decreased under the dark condition. PTX treatment also increased thigmotaxis (increased the anxiety level) of zebrafish larvae. PTZ treatment increased the locomotion of 5 dpf zebrafish larvae under continuous illumination. However, 2 mM PTZ decreased the locomotion and high concentrations of PTZ in our experiment decreased the locomotion of larvae under dark conditions. PTZ treatment increased thigmotaxis, an indicator of increased anxiety levels of zebrafish larvae. When comparing PTX with PTZ, the movements of the larvae were similar between treatments with the two drugs, but PTX caused a higher level of locomotion of 5 dpf zebrafish than PTZ.
[Methodology] The behavioral manifestations to the seizure-inducing drugs PTX (1 μM, 5 μM, 25 μM, 125 μM, or 625 μM) or PTZ (1 mM, 2 mM, 4 mM, 8 mM, or 16 mM) under light-dark conditions were studied using zebrafish larvae at 5 days post-fertilization (dpf). Two behavioral parameters, locomotor activity and thigmotaxis behavior, were analyzed.
[Conclusions] We conclude that PTX treatment increased locomotion and thigmotaxis in 5 dpf zebrafish larvae under continuous illumination and the locomotion of PTX-treated zebrafish was decreased under the dark condition. PTX treatment also increased thigmotaxis (increased the anxiety level) of zebrafish larvae. PTZ treatment increased the locomotion of 5 dpf zebrafish larvae under continuous illumination. However, 2 mM PTZ decreased the locomotion and high concentrations of PTZ in our experiment decreased the locomotion of larvae under dark conditions. PTZ treatment increased thigmotaxis, an indicator of increased anxiety levels of zebrafish larvae. When comparing PTX with PTZ, the movements of the larvae were similar between treatments with the two drugs, but PTX caused a higher level of locomotion of 5 dpf zebrafish than PTZ.