[P3-34] MicroRNA-21contributes to pneumolys induced hyper-permeability of brain microvascular endothelial cells
Increasing evidence indicates that pneumolys (PLY) inducedblood-brain barrier (BBB)opening plays an important role in the pathogenesis ofpneumococcal meningitis. Previously, we found that microRNA-21(miR-21)were upregulated inexperimental pneumococcal meningitis rat, but its effect in regulatingpermeability of brain microvascularendothelial cell lines (BMECs) remains to be evaluated. Here, using a loss-of-function approach, we show that miR-21down-regulation improve PLY induced hyper-permeability of brain microvascular endothelial cells(detecting by transendothelial electrical resistance assay)and F-actin arrangement(detecting by rhodamine-phalloidin staining).Furthermore, this effect is partially associated with modulation of MLC phosphorylationthroughTiaml/Racpathway.Our results suggest that miR-21 contributes to the regulation of BMECspermeabilityafter PLY stimulus. Taken together with previous observations, we propose miR-21 may constitute a potential molecular target for treatment ofpneumococcal meningitis.